Two-photon GCaMP6s imaging at 920 nm desensitizes the red-shifted opsin C1V1 du…

Two-photon GCaMP6s imaging at 920 nm desensitizes the red-shifted opsin C1V1 during all-optical interrogation of mouse layer 2/3 barrel and visual cortex, with the desensitization spreading beyond the targeted cells and worsening at shorter inter-stimulus intervals — a methodological caveat that introduces trial-by-trial variability in all-optical E→E experiments.

{
  "n": 0,
  "doi": "10.1117/1.nph.13.1.015007",
  "claim": "Two-photon GCaMP6s imaging at 920 nm desensitizes the red-shifted opsin C1V1 during all-optical interrogation of mouse layer 2/3 barrel and visual cortex, with the desensitization spreading beyond the targeted cells and worsening at shorter inter-stimulus intervals — a methodological caveat that introduces trial-by-trial variability in all-optical E→E experiments.",
  "cite_key": "Russell2026",
  "evidence": "Documents a previously under-recognised systematic confound in all-optical interrogation, directly bearing on cluster_14's topic of resolution limits and unrecognised biases of E→E tools.",
  "effect_size": null,
  "text_access": "abstract_only",
  "study_system": "Mouse layer 2/3 barrel cortex and visual cortex; GCaMP6s + C1V1 all-optical",
  "argument_role": "supporting",
  "replication_status": "replicated",
  "claim_source_sentence": "We found that two-photon imaging at 920 nm decreases trial-by-trial photostimulation responses in targeted C1V1-expressing neurons-an effect that is exacerbated at shorter inter-stimulus intervals.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [
    "10.1016/j.isci.2024.111152",
    "10.1016/j.xpro.2025.104342"
  ],
  "effect_size_source_sentence": null
}

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