Same-cell EPSC comparison of two excitatory translaminar pathways in mouse S1 b…

{
  "n": null,
  "doi": "10.1038/s41467-024-55783-w",
  "claim": "Same-cell EPSC comparison of two excitatory translaminar pathways in mouse S1 barrel; provides developmental quantitation of E inputs.",
  "cite_key": "VargasOrtiz2025",
  "evidence": "Synchronous neuronal activity is a hallmark of the developing mouse primary somatosensory cortex. While the patterns of synchronous neuronal activity in cortical layer 2/3 have been well described, the source of the robust layer 2/3 activity is still unknown. Using a novel microprism preparation and in vivo 2-photon imaging in neonatal mice, we show that synchronous neuronal activity is organized in barrel columns across layers. Monosynaptic rabies tracing and slice electrophysiology experiments reveal that layer 2/3 pyramidal neurons receive significant layer 5 inputs during the first postnatal week, and silencing layer 5 synaptic outputs results in a significant reduction in spontaneous activity, abnormal sensory-evoked activity and disrupted layer 4-layer 2/3 connectivity. Our results demonstrate that translaminar layer 5-layer 2/3 connectivity plays an important role in synchronizing the developing barrel column to ensure the strengthening of layer 4-layer 2/3 connections, supporting the formation of the canonical cortical organization in barrel cortex.",
  "effect_size": null,
  "text_access": "fulltext",
  "study_system": "Translaminar synchronous neuronal activity is required for columnar synaptic strengthening in the mouse neocortex.",
  "argument_role": "supporting",
  "replication_status": null,
  "claim_source_sentence": "Synaptic strengths of L5-L2/3 versus L4-L2/3 inputs were compared by recording EPSCs in the same L2/3 pyramidal neuron while electrically stimulating L5 or L4 in the same barrel column across three postnatal stages, matching the rabies tracing developmental timeline.",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": null
}

[
  "paper:paper-113803f405c0"
]

Synaptic strengths of L5-L2/3 versus L4-L2/3 inputs were compared by recording EPSCs in the same L2/3 pyramidal neuron while electrically stimulating L5 or L4 in the same barrel column across three postnatal stages, matching the rabies tracing developmental timeline.

[
  {
    "ref": "paper:paper-113803f405c0"
  }
]

{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}

Synchronous neuronal activity is a hallmark of the developing mouse primary somatosensory cortex. While the patterns of synchronous neuronal activity in cortical layer 2/3 have been well described, the source of the robust layer 2/3 activity is still unknown. Using a novel microprism preparation and in vivo 2-photon imaging in neonatal mice, we show that synchronous neuronal activity is organized in barrel columns across layers. Monosynaptic rabies tracing and slice electrophysiology experiments reveal that layer 2/3 pyramidal neurons receive significant layer 5 inputs during the first postnatal week, and silencing layer 5 synaptic outputs results in a significant reduction in spontaneous activity, abnormal sensory-evoked activity and disrupted layer 4-layer 2/3 connectivity. Our results demonstrate that translaminar layer 5-layer 2/3 connectivity plays an important role in synchronizing the developing barrel column to ensure the strengthening of layer 4-layer 2/3 connections, supporting the formation of the canonical cortical organization in barrel cortex.