The genetically encoded voltage indicator ASAP5 reports action potentials in vi…

The genetically encoded voltage indicator ASAP5 reports action potentials in vivo with higher signal-to-noise than previous GEVIs, detects graded subthreshold responses to sensory stimuli in single two-photon trials, and can resolve ~1-mV excitatory postsynaptic potentials in cultured rat or human neurons.

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  "n": 0,
  "doi": "10.1016/j.neuron.2024.08.019",
  "claim": "The genetically encoded voltage indicator ASAP5 reports action potentials in vivo with higher signal-to-noise than previous GEVIs, detects graded subthreshold responses to sensory stimuli in single two-photon trials, and can resolve ~1-mV excitatory postsynaptic potentials in cultured rat or human neurons.",
  "cite_key": "Hao2024",
  "evidence": "Voltage indicator advance enabling readout of subthreshold and unitary synaptic events — addresses cluster_14 'subthreshold network activity' methodological gap.",
  "effect_size": "~1-mV EPSPs resolvable in single trials",
  "text_access": "abstract_only",
  "study_system": "Mouse cortex in vivo + cultured rat/human neurons; ASAP5 GEVI",
  "argument_role": "supporting",
  "replication_status": "single-study",
  "claim_source_sentence": "ASAP5 reported action potentials (APs) in vivo with higher signal-to-noise ratios than previous GEVIs and successfully detected graded and subthreshold responses to sensory stimuli in single two-photon trials.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": "In cultured rat or human neurons, somatic ASAP5 reported synaptic events propagating centripetally and could detect ∼1-mV EPSPs."
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15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon

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