- claim_text
Time-multiplexed pulsed excitation in a two-photon all-optical system can separate fluorescence signal from photostimulation crosstalk for both calcium and voltage indicators in vivo, addressing a specific resolution limit of E→E all-optical interrogation.
- raw_fields
{
"n": 0,
"doi": "10.1364/boe.521047",
"claim": "Time-multiplexed pulsed excitation in a two-photon all-optical system can separate fluorescence signal from photostimulation crosstalk for both calcium and voltage indicators in vivo, addressing a specific resolution limit of E→E all-optical interrogation.",
"cite_key": "Liu2024c",
"evidence": "Documents both the existence of a known imaging/photostimulation crosstalk confound and an explicit hardware-level mitigation — relevant to cluster_14's resolution-limit discussion.",
"effect_size": null,
"text_access": "abstract_only",
"study_system": "In vivo two-photon all-optical physiology system",
"argument_role": "supporting",
"replication_status": "single-study",
"claim_source_sentence": "We demonstrate the efficacy of our system in eliminating crosstalk signals from photostimulation and evaluate its influence on both genetically encoded calcium indicators (GECIs) and genetically encoded voltage indicators (GEVIs) through <i>in vivo</i> experiments.",
"source_provenance_status": "non_substring_match",
"replication_evidence_dois": [],
"effect_size_source_sentence": null
}- source_refs
[
"paper:paper-f2e43c8255e5"
]
- source_span
We demonstrate the efficacy of our system in eliminating crosstalk signals from photostimulation and evaluate its influence on both genetically encoded calcium indicators (GECIs) and genetically encoded voltage indicators (GEVIs) through <i>in vivo</i> experiments.
- evidence_refs
[
{
"ref": "paper:paper-f2e43c8255e5"
}
]- source_policy
{
"mode": "public_source_pointer_with_short_context",
"notes": [
"Local review repositories are read-only inputs.",
"SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
],
"source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
"source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}