Controlled in vivo experiments in layer-2 mouse neocortex varying only the axia…

Controlled in vivo experiments in layer-2 mouse neocortex varying only the axial extent of the photostimulation point spread function show that optical resolution is not always the limiting factor for the spatial precision of two-photon optogenetic photostimulation, identifying other factors that must be improved to maximize precision.

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  "n": 0,
  "doi": "10.1117/1.nph.11.1.015006",
  "claim": "Controlled in vivo experiments in layer-2 mouse neocortex varying only the axial extent of the photostimulation point spread function show that optical resolution is not always the limiting factor for the spatial precision of two-photon optogenetic photostimulation, identifying other factors that must be improved to maximize precision.",
  "cite_key": "Lees2024",
  "evidence": "Quantifies which optical factors actually limit single-cell stimulation precision in mouse cortex — central to cluster_14 all-optical methodological-limits discussion.",
  "effect_size": null,
  "text_access": "fulltext",
  "study_system": "Mouse layer 2 neocortex; two-photon photostimulation + calcium imaging",
  "argument_role": "supporting",
  "replication_status": "single-study",
  "claim_source_sentence": "We sought to answer one specific aspect of these general queries: how much optical resolution is necessary? We filled this gap in knowledge by performing a controlled experiment varying only the optical resolution while holding all other factors constant.",
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We sought to answer one specific aspect of these general queries: how much optical resolution is necessary? We filled this gap in knowledge by performing a controlled experiment varying only the optical resolution while holding all other factors constant.

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