Comparative single-nucleus transcriptomic and epigenomic profiling of >450,000…

Comparative single-nucleus transcriptomic and epigenomic profiling of >450,000 cells in mouse, marmoset and human primary motor cortex finds a 5:1 glutamatergic:GABAergic ratio in mouse vs 2:1 in human, with mouse M1 containing proportionally more L6 corticothalamic and L5 extratelencephalic neurons than primates, suggesting evolutionary scaling of long-range cortical projection-type abundance.

{
  "n": 450000,
  "doi": "10.1038/s41586-021-03465-8",
  "claim": "Comparative single-nucleus transcriptomic and epigenomic profiling of >450,000 cells in mouse, marmoset and human primary motor cortex finds a 5:1 glutamatergic:GABAergic ratio in mouse vs 2:1 in human, with mouse M1 containing proportionally more L6 corticothalamic and L5 extratelencephalic neurons than primates, suggesting evolutionary scaling of long-range cortical projection-type abundance.",
  "cite_key": "Bakken2021",
  "evidence": "High-throughput snRNA-seq and snATAC-seq of >450,000 nuclei from mouse, marmoset and human M1 with cross-species alignment.",
  "effect_size": "quantitative — 5:1 (mouse) vs 2:1 (human) E:I ratio; mouse has higher fraction of L5 ET and L6 CT than primates",
  "text_access": "fulltext",
  "study_system": "mouse, marmoset, human primary motor cortex (M1), single-nucleus profiling",
  "argument_role": "supporting",
  "replication_status": "single_study",
  "claim_source_sentence": "the ratio of glutamatergic to GABAergic neurons varies from 2:1 in humans to 3:1 in marmosets and 5:1 in mice. The relative proportions of GABAergic subclasses and types are similar across species, suggesting a global increase in GABAergic types. As described previously, we observed proportionally more L2 and L3 intratelencephalic neurons in humans, representing a selective increase in the number of neurons projecting to other parts of the cortex, presumably to facilitate greater corticocortical communication. Humans and marmosets have proportionally fewer L6 corticothalamic and L5 extratelencephalic neurons",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": "the ratio of glutamatergic to GABAergic neurons varies from 2:1 in humans to 3:1 in marmosets and 5:1 in mice"
}

[
  "paper:paper-pm-34616062"
]

the ratio of glutamatergic to GABAergic neurons varies from 2:1 in humans to 3:1 in marmosets and 5:1 in mice. The relative proportions of GABAergic subclasses and types are similar across species, suggesting a global increase in GABAergic types. As described previously, we observed proportionally more L2 and L3 intratelencephalic neurons in humans, representing a selective increase in the number of neurons projecti...

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