A Drop-seq atlas of 690,000 single cells across nine adult mouse-brain regions,…

A Drop-seq atlas of 690,000 single cells across nine adult mouse-brain regions, including cortex, defined 565 transcriptionally distinct cell groups and revealed glutamatergic-neuron specialization across cortical regions — providing the cell-population scaffolding on which mouse-cortex E→E circuit synthesis is built.

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  "claim": "A Drop-seq atlas of 690,000 single cells across nine adult mouse-brain regions, including cortex, defined 565 transcriptionally distinct cell groups and revealed glutamatergic-neuron specialization across cortical regions — providing the cell-population scaffolding on which mouse-cortex E→E circuit synthesis is built.",
  "cite_key": "Saunders2018",
  "evidence": "690K-cell Drop-seq atlas; 565 cell groups; cross-region cortical glutamatergic specialization.",
  "effect_size": "690,000 cells profiled; 565 transcriptionally distinct groups; 9 brain regions",
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To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells sampled from 9 regions of the adult mouse brain. We identified 565 transcriptionally distinct groups of cells using computational approaches developed to distinguish biological from technical signals.

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15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon

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