MAPseq barcoded axonal tracing of 591 individual mouse V1 neurons showed that s…

MAPseq barcoded axonal tracing of 591 individual mouse V1 neurons showed that single intracortical projection neurons targeted at least 18 cortical and subcortical areas, mostly in non-random multi-area combinations — directly refuting one-neuron-one-target models of mouse-cortex E→E information transfer.

{
  "n": 591,
  "doi": "10.1038/nature26159",
  "claim": "MAPseq barcoded axonal tracing of 591 individual mouse V1 neurons showed that single intracortical projection neurons targeted at least 18 cortical and subcortical areas, mostly in non-random multi-area combinations — directly refuting one-neuron-one-target models of mouse-cortex E→E information transfer.",
  "cite_key": "Han2018",
  "evidence": "591 V1 neurons traced; ≥18 target areas; non-random multi-area combinations.",
  "effect_size": "591 single neurons; ≥18 target cortical/subcortical areas; dominant mode is multi-target",
  "text_access": "abstract_only",
  "study_system": "Mouse V1, MAPseq barcoded single-neuron projection mapping",
  "argument_role": "supporting",
  "replication_status": "single-study",
  "claim_source_sentence": "Here we determine the projection patterns of 591 individual neurons in the mouse primary visual cortex using whole-brain fluorescence-based axonal tracing and high-throughput DNA sequencing of genetically barcoded neurons (MAPseq). Projections were highly diverse and divergent, collectively targeting at least 18 cortical and subcortical areas.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": "Most neurons targeted multiple cortical areas, often in non-random combinations, suggesting that sub-classes of intracortical projection neurons exist."
}

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Here we determine the projection patterns of 591 individual neurons in the mouse primary visual cortex using whole-brain fluorescence-based axonal tracing and high-throughput DNA sequencing of genetically barcoded neurons (MAPseq). Projections were highly diverse and divergent, collectively targeting at least 18 cortical and subcortical areas.

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15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon

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