- claim_text
CUBIC is a chemical-cocktail whole-brain clearing protocol that supports rapid single-cell-resolution imaging by single-photon microscopy of intact adult mouse brain, providing the methodological substrate for whole-cortex multiplexed counterstaining of cortical activity markers.
- raw_fields
{
"n": 0,
"doi": "10.1016/j.cell.2014.03.042",
"claim": "CUBIC is a chemical-cocktail whole-brain clearing protocol that supports rapid single-cell-resolution imaging by single-photon microscopy of intact adult mouse brain, providing the methodological substrate for whole-cortex multiplexed counterstaining of cortical activity markers.",
"cite_key": "Susaki2014",
"evidence": "Chemical-cocktail clearing of intact adult mouse brain; single-cell-resolution whole-brain imaging with single-photon microscopy; quantification of stimulus-evoked nuclear activity markers.",
"effect_size": "single-cell resolution whole-brain imaging in cleared adult mouse",
"text_access": "abstract_only",
"study_system": "Whole adult mouse brain (clearing protocol)",
"argument_role": "supporting",
"replication_status": "method-paper",
"claim_source_sentence": "we performed comprehensive chemical screening to develop a whole-brain clearing and imaging method, termed CUBIC (clear, unobstructed brain imaging cocktails and computational analysis). CUBIC is a simple and efficient method involving the immersion of brain samples in chemical mixtures containing aminoalcohols, which enables rapid whole-brain imaging with single-photon excitation microscopy.",
"source_provenance_status": "non_substring_match",
"replication_evidence_dois": [],
"effect_size_source_sentence": "enable the visualization and quantification of neural activities induced by environmental stimulation."
}- source_refs
[
"paper:paper-622bfe2cb817"
]
- source_span
we performed comprehensive chemical screening to develop a whole-brain clearing and imaging method, termed CUBIC (clear, unobstructed brain imaging cocktails and computational analysis). CUBIC is a simple and efficient method involving the immersion of brain samples in chemical mixtures containing aminoalcohols, which enables rapid whole-brain imaging with single-photon excitation microscopy.
- evidence_refs
[
{
"ref": "paper:paper-622bfe2cb817"
}
]- section_title
15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon
- source_policy
{
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"notes": [
"Local review repositories are read-only inputs.",
"SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
],
"source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
"source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
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