Version history

{
  "scope": "adult mouse primary visual cortex (V1) supragranular layers",
  "claim_text": "Multi-spot two-photon optogenetic stimulation combined with multicellular patch-clamp recordings in mouse V1 enables monosynaptic connectivity measurement from single Cre-defined presynaptic neurons across cortical layers, with optogenetic-vs-paired-recording validation showing concordant intralaminar and translaminar E→E connection probabilities and amplitudes for L2/3 targets.",
  "raw_fields": {
    "n": 0,
    "doi": "10.7554/elife.71103",
    "claim": "Multi-spot two-photon optogenetic stimulation combined with multicellular patch-clamp recordings in mouse V1 enables monosynaptic connectivity measurement from single Cre-defined presynaptic neurons across cortical layers, with optogenetic-vs-paired-recording validation showing concordant intralaminar and translaminar E→E connection probabilities and amplitudes for L2/3 targets.",
    "cite_key": "Hage2022",
    "evidence": "Two-photon spatial-light-modulator optogenetic stimulation paired with quadruple whole-cell recording in mouse V1 slices, validated against conventional pairwise patch.",
    "effect_size": "qualitative — high-throughput inter- and intralaminar connectivity measurement in mouse V1",
    "text_access": "fulltext",
    "study_system": "adult mouse primary visual cortex (V1) supragranular layers",
    "argument_role": "supporting",
    "replication_status": "single_study",
    "claim_source_sentence": "we combined spatially precise optogenetic stimulation with multicellular recording to deeply characterize intralaminar and translaminar monosynaptic connections to supragranular (L2/3) neurons in the mouse visual cortex. The reliability and specificity of multiphoton optogenetic stimulation were measured across multiple Cre lines, and measurements of connectivity were verified by comparison to paired r",
    "source_provenance_status": "ok",
    "replication_evidence_dois": [],
    "effect_size_source_sentence": "The reliability and specificity of multiphoton optogenetic stimulation were measured across multiple Cre lines, and measurements of connectivity were verified by comparison to paired r"
  },
  "section_id": "section_07",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_07_evidence_package.json",
  "effect_size": "qualitative — high-throughput inter- and intralaminar connectivity measurement in mouse V1",
  "review_repo": "ComputationalReviewRecurrence",
  "section_ref": "wiki_page:computationalreviewrecurrence-07-celltype-motifs",
  "source_kind": "review_finding",
  "source_path": "evidence/section_07_evidence_package.json",
  "source_refs": [
    "paper:paper-14e5fb681943"
  ],
  "source_span": "we combined spatially precise optogenetic stimulation with multicellular recording to deeply characterize intralaminar and translaminar monosynaptic connections to supragranular (L2/3) neurons in the mouse visual cortex. The reliability and specificity of multiphoton optogenetic stimulation were measured across multiple Cre lines, and measurements of connectivity were verified by comparison to paired r",
  "study_system": "adult mouse primary visual cortex (V1) supragranular layers",
  "evidence_refs": [
    {
      "ref": "paper:paper-14e5fb681943"
    }
  ],
  "section_title": "7. Cell-type-specific E→E motifs in mouse — IT vs PT vs CT pyramidal projection classes; L5 thick-tufted recurrence; Patch-seq and Allen mouse-cortex taxonomy intersections; transcriptomic-type-specific connectivity",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
  },
  "evidence_summary": "Two-photon spatial-light-modulator optogenetic stimulation paired with quadruple whole-cell recording in mouse V1 slices, validated against conventional pairwise patch.",
  "review_bundle_ref": "analysis_bundle:ab-d9c479db9be9",
  "replication_status": "single_study",
  "review_package_ref": "analysis_bundle:ab-d9c479db9be9",
  "source_artifact_ref": "wiki_page:computationalreviewrecurrence-07-celltype-motifs",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_07_evidence_package.json",
  "commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}