JEDI-2P, an indicator engineered specifically for 2P microscopy, supports susta…

JEDI-2P, an indicator engineered specifically for 2P microscopy, supports sustained voltage recording from individual cortical neurons in awake mice for >30 minutes and detects spikes beyond 400 µm depth using ULoVE random-access scanning — bounding the present-day depth and duration of 2P voltage imaging in cortex.

{
  "n": 0,
  "doi": "10.1016/j.cell.2022.07.013",
  "claim": "JEDI-2P, an indicator engineered specifically for 2P microscopy, supports sustained voltage recording from individual cortical neurons in awake mice for >30 minutes and detects spikes beyond 400 µm depth using ULoVE random-access scanning — bounding the present-day depth and duration of 2P voltage imaging in cortex.",
  "cite_key": "Liu2022a",
  "evidence": "Quantifies the leading-edge time and depth window of 2P voltage imaging; complements the 12-neuron / 300 µm constraint from optical-constraints paper.",
  "effect_size": ">30 min recording duration; spike detection at >400 µm depth (ULoVE)",
  "text_access": "abstract_only",
  "study_system": "Awake behaving mouse cortex; JEDI-2P GEVI under 2P microscopy",
  "argument_role": "supporting",
  "replication_status": "replicated",
  "claim_source_sentence": "JEDI-2P can also optically record the voltage dynamics of individual cortical neurons in awake behaving mice for more than 30 min using both resonant-scanning and ULoVE random-access microscopy.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [
    "10.1117/1.nph.11.3.035007",
    "10.1126/sciadv.adp5763"
  ],
  "effect_size_source_sentence": "Finally, ULoVE recording of JEDI-2P can robustly detect spikes at depths exceeding 400 μm and report voltage correlations in pairs of neurons."
}

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]

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    "ref": "paper:paper-07e278a78498"
  }
]

15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon

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