Version history

{
  "scope": "Nkx2.1-Cre conditional Dot1l KO mouse",
  "claim_text": "Cell-autonomous deletion of the histone methyltransferase DOT1L from Nkx2.1+ MGE interneuron precursors reduces overall cortical-interneuron numbers from P0, with a disproportionate decrease in parvalbumin (PV) INs. Mitotic cell counts at E14.5 are reduced, indicating premature cell-cycle exit; postmitotic effects on differentiation/migration also contribute, identifying DOT1L as an MGE-IN epigenetic regulator.",
  "raw_fields": {
    "n": null,
    "doi": "10.1093/cercor/bhad281",
    "claim": "Cell-autonomous deletion of the histone methyltransferase DOT1L from Nkx2.1+ MGE interneuron precursors reduces overall cortical-interneuron numbers from P0, with a disproportionate decrease in parvalbumin (PV) INs. Mitotic cell counts at E14.5 are reduced, indicating premature cell-cycle exit; postmitotic effects on differentiation/migration also contribute, identifying DOT1L as an MGE-IN epigenetic regulator.",
    "title": null,
    "cite_key": "Cheffer2023",
    "evidence": "Cerebral Cortex 2023. Method: Nkx2.1-Cre; Dot1l-flox conditional KO; histology + IHC at E14.5/P0/postnatal stages.",
    "effect_size": null,
    "figure_data": null,
    "text_access": "abstract_only",
    "key_entities": [
      "DOT1L",
      "histone methyltransferase",
      "Nkx2.1",
      "MGE",
      "PV",
      "parvalbumin",
      "cell-cycle exit",
      "E14.5",
      "P0",
      "interneuron",
      "epigenetic"
    ],
    "study_system": "Nkx2.1-Cre conditional Dot1l KO mouse",
    "_source_cluster": "cluster_02_development_lineage",
    "replication_status": "single_study",
    "_source_cluster_index": 141,
    "claim_source_sentence": "Deletion of Dot1l in Nkx2.1-expressing interneuron precursor cells results in an overall reduction and altered distribution of GABAergic interneurons in the CP from postnatal day 0 onwards. We observed an altered proportion of GABAergic interneurons in the cortex, with a significant decrease in parvalbumin-expressing interneurons. Moreover, a decreased number of mitotic cells at the embryonic day E14.5 was observed upon Dot1l deletion.",
    "replication_evidence_dois": [],
    "effect_size_source_sentence": null
  },
  "section_id": "section_03",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP/blob/95e761177f7d2ec565983d3307c14ec238f9677c/evidence/section_03_evidence_package.json",
  "effect_size": null,
  "review_repo": "ComputationalReviewVIP",
  "section_ref": "wiki_page:computationalreviewvip-03-development",
  "source_kind": "review_finding",
  "source_path": "evidence/section_03_evidence_package.json",
  "source_refs": [
    "paper:paper-616950b87f7f"
  ],
  "source_span": "Deletion of Dot1l in Nkx2.1-expressing interneuron precursor cells results in an overall reduction and altered distribution of GABAergic interneurons in the CP from postnatal day 0 onwards. We observed an altered proportion of GABAergic interneurons in the cortex, with a significant decrease in parvalbumin-expressing interneurons. Moreover, a decreased number of mitotic cells at the embryonic day E14.5 was observed...",
  "study_system": "Nkx2.1-Cre conditional Dot1l KO mouse",
  "evidence_refs": [
    {
      "ref": "paper:paper-616950b87f7f"
    }
  ],
  "section_title": "Developmental Origins and Postnatal Maturation",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "95e761177f7d2ec565983d3307c14ec238f9677c",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP"
  },
  "evidence_summary": "Cerebral Cortex 2023. Method: Nkx2.1-Cre; Dot1l-flox conditional KO; histology + IHC at E14.5/P0/postnatal stages.",
  "review_bundle_ref": "analysis_bundle:ab-2ce40c33e827",
  "replication_status": "single_study",
  "review_package_ref": "analysis_bundle:ab-2ce40c33e827",
  "source_artifact_ref": "wiki_page:computationalreviewvip-03-development",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP/blob/95e761177f7d2ec565983d3307c14ec238f9677c/evidence/section_03_evidence_package.json",
  "commit_sha": "95e761177f7d2ec565983d3307c14ec238f9677c",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP"
}