MAPseq reconstruction of single-cell axonal projections from 591 individual mou…

MAPseq reconstruction of single-cell axonal projections from 591 individual mouse V1 neurons shows that V1 collectively targets at least 18 cortical and subcortical areas, most individual neurons project to multiple cortical areas in non-random combinations rather than to a single area, and the population partitions into sub-classes of intracortical projection neurons defined by their target-combination preference.

{
  "n": 591,
  "doi": "10.1038/nature26159",
  "claim": "MAPseq reconstruction of single-cell axonal projections from 591 individual mouse V1 neurons shows that V1 collectively targets at least 18 cortical and subcortical areas, most individual neurons project to multiple cortical areas in non-random combinations rather than to a single area, and the population partitions into sub-classes of intracortical projection neurons defined by their target-combination preference.",
  "cite_key": "Han2018",
  "evidence": "MAPseq single-cell axonal-barcode sequencing of 591 V1 neurons with whole-brain dissection of cortical and subcortical target areas in mouse.",
  "effect_size": "quantitative — 591 V1 neurons, ≥18 targets; non-random multi-target combinations",
  "text_access": "fulltext",
  "study_system": "adult mouse primary visual cortex (V1) projection neurons",
  "argument_role": "supporting",
  "replication_status": "independently_replicated",
  "claim_source_sentence": "Projections were highly diverse and divergent, collectively targeting at least 18 cortical and subcortical areas. Most neurons target multiple cortical areas, often in non-random combinations, suggesting the existence of sub-classes of intracortical projection neurons. Thus the dominant mode of intracortical information transfer is not based on 'one neuron – one target area' mapping",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [
    "10.1101/2025.06.25.661405",
    "10.1038/s41586-021-04020-1"
  ],
  "effect_size_source_sentence": "591 individual neurons in mouse primary visual cortex (V1)... collectively targeting at least 18 cortical and subcortical areas"
}

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  "paper:paper-236d0f50c2dd"
]

Projections were highly diverse and divergent, collectively targeting at least 18 cortical and subcortical areas. Most neurons target multiple cortical areas, often in non-random combinations, suggesting the existence of sub-classes of intracortical projection neurons. Thus the dominant mode of intracortical information transfer is not based on 'one neuron – one target area' mapping

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