Mouse S1 columnar synchronous network activity is driven by L5→L2/3 excitatory…

{
  "n": null,
  "doi": "10.1038/s41467-024-55783-w",
  "claim": "Mouse S1 columnar synchronous network activity is driven by L5→L2/3 excitatory inputs early in development.",
  "cite_key": "VargasOrtiz2025",
  "evidence": "Synchronous neuronal activity is a hallmark of the developing mouse primary somatosensory cortex. While the patterns of synchronous neuronal activity in cortical layer 2/3 have been well described, the source of the robust layer 2/3 activity is still unknown. Using a novel microprism preparation and in vivo 2-photon imaging in neonatal mice, we show that synchronous neuronal activity is organized in barrel columns across layers. Monosynaptic rabies tracing and slice electrophysiology experiments reveal that layer 2/3 pyramidal neurons receive significant layer 5 inputs during the first postnatal week, and silencing layer 5 synaptic outputs results in a significant reduction in spontaneous activity, abnormal sensory-evoked activity and disrupted layer 4-layer 2/3 connectivity. Our results demonstrate that translaminar layer 5-layer 2/3 connectivity plays an important role in synchronizing the developing barrel column to ensure the strengthening of layer 4-layer 2/3 connections, supporting the formation of the canonical cortical organization in barrel cortex.",
  "effect_size": null,
  "text_access": "fulltext",
  "study_system": "Translaminar synchronous neuronal activity is required for columnar synaptic strengthening in the mouse neocortex.",
  "argument_role": "supporting",
  "replication_status": null,
  "claim_source_sentence": "Reducing L5 synaptic inputs to L2/3 pyramidal neurons by expressing tetanus toxin light chain (TeLC) or inhibitory Gi-DREADD specifically in L5 pyramidal neurons led to significant reduction in L2/3 columnar SNAs and a lower number of sensory stimulus–responsive cells in mouse S1.",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": null
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[
  "paper:paper-113803f405c0"
]

Reducing L5 synaptic inputs to L2/3 pyramidal neurons by expressing tetanus toxin light chain (TeLC) or inhibitory Gi-DREADD specifically in L5 pyramidal neurons led to significant reduction in L2/3 columnar SNAs and a lower number of sensory stimulus–responsive cells in mouse S1.

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Synchronous neuronal activity is a hallmark of the developing mouse primary somatosensory cortex. While the patterns of synchronous neuronal activity in cortical layer 2/3 have been well described, the source of the robust layer 2/3 activity is still unknown. Using a novel microprism preparation and in vivo 2-photon imaging in neonatal mice, we show that synchronous neuronal activity is organized in barrel columns across layers. Monosynaptic rabies tracing and slice electrophysiology experiments reveal that layer 2/3 pyramidal neurons receive significant layer 5 inputs during the first postnatal week, and silencing layer 5 synaptic outputs results in a significant reduction in spontaneous activity, abnormal sensory-evoked activity and disrupted layer 4-layer 2/3 connectivity. Our results demonstrate that translaminar layer 5-layer 2/3 connectivity plays an important role in synchronizing the developing barrel column to ensure the strengthening of layer 4-layer 2/3 connections, supporting the formation of the canonical cortical organization in barrel cortex.