Methods paper: 2-4 cell two-photon-targeted patch monosynaptic connectivity in…

{
  "n": null,
  "doi": "10.3389/fnsyn.2019.00015",
  "claim": "Methods paper: 2-4 cell two-photon-targeted patch monosynaptic connectivity in mouse V1 L1-3.",
  "cite_key": "Jouhanneau2019",
  "evidence": "Although we know a great deal about monosynaptic connectivity, transmission and integration in the mammalian nervous system fromstudies, very little is known. This is partly because it is technically difficult to evoke action potentials and simultaneously record small amplitude subthreshold responses in closely (<150 &#x3bc;m) located pairs of neurons. To address this, we have developedtwo-photon targeted multiple (2-4) whole-cell patch clamp recordings of nearby neurons in superficial cortical layers 1-3. Here, we describe a step-by-step guide to this approach in the anesthetized mouse primary somatosensory cortex, including: the design of the setup, surgery, preparation of pipettes, targeting and acquisition of multiple whole-cell recordings, as well asandhistology. The procedure takes ~4 h from start of surgery to end of recording and allows examinations both into the electrophysiological features of unitary excitatory and inhibitory monosynaptic inputs during different brain states as well as the synaptic mechanisms of correlated neuronal activity.",
  "effect_size": null,
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  "study_system": "Multiple Two-Photon Targeted Whole-Cell Patch-Clamp Recordings From Monosynaptically Connected Neurons.",
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  "claim_source_sentence": "We have developed two-photon targeted multiple (2-4) whole-cell patch clamp recordings of nearby neurons in superficial cortical layers 1-3 [in mouse V1].",
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Although we know a great deal about monosynaptic connectivity, transmission and integration in the mammalian nervous system fromstudies, very little is known. This is partly because it is technically difficult to evoke action potentials and simultaneously record small amplitude subthreshold responses in closely (<150 &#x3bc;m) located pairs of neurons. To address this, we have developedtwo-photon targeted multiple (2-4) whole-cell patch clamp recordings of nearby neurons in superficial cortical layers 1-3. Here, we describe a step-by-step guide to this approach in the anesthetized mouse primary somatosensory cortex, including: the design of the setup, surgery, preparation of pipettes, targeting and acquisition of multiple whole-cell recordings, as well asandhistology. The procedure takes ~4 h from start of surgery to end of recording and allows examinations both into the electrophysiological features of unitary excitatory and inhibitory monosynaptic inputs during different brain states as well as the synaptic mechanisms of correlated neuronal activity.