Hrvatin et al. (2019) developed PESCA, a scalable platform combining ATAC-seq w…

Hrvatin et al. (2019) developed PESCA, a scalable platform combining ATAC-seq with viral barcoding to identify cell-type-specific enhancers, generating viral reagents that target SST cortical interneurons and providing transferable methodology for VIP and PV subtype-specific genetic access.

{
  "n": null,
  "doi": "10.7554/elife.48089",
  "claim": "Hrvatin et al. (2019) developed PESCA, a scalable platform combining ATAC-seq with viral barcoding to identify cell-type-specific enhancers, generating viral reagents that target SST cortical interneurons and providing transferable methodology for VIP and PV subtype-specific genetic access.",
  "title": null,
  "cite_key": "Hrvatin2019",
  "evidence": "ATAC-seq on FACS-purified PV, SST, VIP cortical nuclei → 323,369 putative GREs → 287 SST-specific candidates screened via AAV in mouse V1; produces enhancer-AAV toolkit applicable to VIP types as used in subsequent Patch-seq studies (Jorstad 2023).",
  "effect_size": null,
  "text_access": "fulltext",
  "study_system": "mouse V1 cortex; ATAC-seq + AAV GRE screening",
  "target_section": "section_11",
  "_source_cluster": "cluster_01_molecular_taxonomy",
  "replication_status": "single_study",
  "_source_cluster_index": 39,
  "claim_source_sentence": "We validated the efficacy of PESCA in the murine primary visual cortex by identifying GREs that confine AAV expression to somatostatin (SST)-expressing interneurons and showed that these vectors can be used to modulate neuronal activity selectively in SST neurons. These datasets can be used as a resource to identify putative gene regulatory elements as candidates for driving cell-type-specific gene expression for the numerous subtypes of SST, PV or VIP-expressing intraneurons across diverse cortical regions."
}

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  "paper:paper-04bb600a01a5"
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We validated the efficacy of PESCA in the murine primary visual cortex by identifying GREs that confine AAV expression to somatostatin (SST)-expressing interneurons and showed that these vectors can be used to modulate neuronal activity selectively in SST neurons. These datasets can be used as a resource to identify putative gene regulatory elements as candidates for driving cell-type-specific gene expression for th...

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ATAC-seq on FACS-purified PV, SST, VIP cortical nuclei → 323,369 putative GREs → 287 SST-specific candidates screened via AAV in mouse V1; produces enhancer-AAV toolkit applicable to VIP types as used in subsequent Patch-seq studies (Jorstad 2023).