iDISCO is a whole-mount immunolabeling-plus-volume-imaging protocol compatible…

iDISCO is a whole-mount immunolabeling-plus-volume-imaging protocol compatible with 28 tested antibodies that permits visualization of single degenerating axons in adult brain — the protocol that underlies most subsequent cleared-tissue whole-mouse-cortex axon-tracing work.

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  "doi": "10.1016/j.cell.2014.10.010",
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  "cite_key": "Renier2014",
  "evidence": "Tested with 28 antibodies; single-axon resolution in adult mouse brain; whole-mount volumes.",
  "effect_size": "28 antibodies validated; single-axon resolution in cleared adult mouse brain",
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  "claim_source_sentence": "We describe a simple, rapid, and inexpensive method, iDISCO, that permits whole-mount immunolabeling with volume imaging of large cleared samples ranging from perinatal mouse embryos to adult organs, such as brains or kidneys. iDISCO is modeled on classical histology techniques, facilitating translation of section staining assays to intact tissues, as evidenced by compatibility with 28 antibodies to both endogenous antigens and transgenic reporters like GFP.",
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  "effect_size_source_sentence": "It also permitted imaging of single degenerating axons in adult brain and the first visualization of cleaved Caspase-3 in degenerating embryonic sensory axons in vivo, even single axons."
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We describe a simple, rapid, and inexpensive method, iDISCO, that permits whole-mount immunolabeling with volume imaging of large cleared samples ranging from perinatal mouse embryos to adult organs, such as brains or kidneys. iDISCO is modeled on classical histology techniques, facilitating translation of section staining assays to intact tissues, as evidenced by compatibility with 28 antibodies to both endogenous...

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15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon

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