The best development plan is a temporal map of autophagy after irradiation in primary human brain pericytes: 6 h, 24 h, 72 h, and senescence endpoints. That can separate initiation defects from clearance defects and reveal whether mitophagy failure is a primary driver or a secondary consequence.
Lysosome and mitophagy programs both offer tractable intervention hooks. If acidification failure dominates, TFEB activators or lysosome-repair strategies become attractive; if mitophagy dominates, mitochondrial QC enhancers are the cleaner path. For translational relevance, the most important bridge is whether the same autophagy lesion correlates with BBB dysfunction markers and inflammatory secretome changes, not simply with senescence staining.