What resolves this contention: Method (in vivo vs slice) and slice-distance bia…

What resolves this contention: Method (in vivo vs slice) and slice-distance bias can produce systematically different P(connection) estimates for the same nominal mouse L2/3 pyramidal-pyramidal target population; conflict over whether slice recordings inflate connectivity by selecting closer surviving axons. / In vivo paired recordings in mouse L2 barrel cortex find ~6.7% connection probability between nearby layer 2 pyramidal neurons. / In vitro multi-cell recordings in the mouse S1 C2 barrel column report substantially higher within-L2/3 connection probabilities (~20%+ at near distances, layer-dependent).