What resolves this contention: Whether Ntsr1-Cre labels a homogeneous L6-CT cla…

What resolves this contention: Whether Ntsr1-Cre labels a homogeneous L6-CT class. The Cre-line characterization paper treats Ntsr1-Cre+ neurons as a clean projection-class identifier (CT only, ~90% penetrance), while the awake-recording paper shows the same Cre-defined population contains functionally distinct subpopulations (whisking-modulated, suppressed, sparse, silent) — i.e., a single Cre line does not isolate a functionally uniform projection class. / We conclude that Cre-expression is limited to a specific type of pyramidal neuron: CT. However, it appears as not all CT neurons are Cre-expressing; there are indications that the penetrance of the gene is about 90%. We demonstrate the utility of assigning a specific identity to individual neurons by determining that the CT neurons are potently modulated by acetylcholine acting on both nicotinic and muscarinic acetylcholine receptors. / Their spiking patterns were