{
"papers": [
{
"n": 4200,
"doi": "10.1016/j.cell.2020.09.057",
"value": "4200",
"method": "10x Chromium scRNA-seq + patch-clamp",
"metric": "Number of GABAergic interneurons profiled (transcriptomics)",
"n_analyzed": "4200",
"ci_or_error": null,
"text_access": "fulltext",
"n_definition": "cells profiled with scRNA-seq",
"scope_region": "visual cortex only",
"study_system": "mouse visual cortex",
"taxonomic_level": "28 met-types",
"scope_population": "GABAergic interneurons only",
"value_source_sentence": "we characterized the transcriptomes and intrinsic physiological properties of over 4,200 mouse visual cortical GABAergic interneurons",
"experimental_conditions": "acute brain slices, Patch-seq"
},
{
"n": 1300000,
"doi": "10.1016/j.cell.2021.04.021",
"value": "1300000",
"method": "10x Chromium scRNA-seq",
"metric": "Number of cells profiled (transcriptomics)",
"n_analyzed": "1300000",
"ci_or_error": null,
"text_access": "fulltext",
"n_definition": "cells profiled with scRNA-seq",
"scope_region": "isocortex and hippocampal formation",
"study_system": "mouse isocortex and hippocampal formation",
"taxonomic_level": "subclass level (Pvalb = 1 subclass with 3 supertypes)",
"scope_population": "all cell types",
"value_source_sentence": "We profiled ~1.3 million cells covering the entire adult mouse isocortex and HPF",
"experimental_conditions": "10x Chromium scRNA-seq"
},
{
"n": 11300000,
"doi": "10.1038/s41586-023-06812-z",
"value": "11300000",
"method": "10x scRNA-seq + MERFISH spatial transcriptomics",
"metric": "Number of cells profiled (scRNA-seq + MERFISH)",
"n_analyzed": "11300000",
"ci_or_error": null,
"text_access": "abstract_only",
"n_definition": "cells profiled across both modalities",
"scope_region": "whole brain",
"study_system": "whole adult mouse brain",
"taxonomic_level": "5,200 clusters in whole brain",
"scope_population": "all cell types",
"value_source_sentence": "a single-cell RNA-sequencing (scRNA-seq) dataset of ~7 million cells profiled, and a spatially resolved transcriptomic dataset of ~4.3 million cells using MERFISH",
"experimental_conditions": "scRNA-seq + MERFISH"
},
{
"n": 611423,
"doi": "10.1101/2024.06.18.599583",
"value": "611423",
"method": "scRNA-seq",
"metric": "Number of GABAergic cells profiled",
"n_analyzed": "611423",
"ci_or_error": null,
"text_access": "fulltext",
"n_definition": "GABAergic neuronal cells profiled",
"scope_region": "whole telencephalon",
"study_system": "mouse whole telencephalon",
"taxonomic_level": "1,051 GABAergic clusters in 52 subclasses",
"scope_population": "GABAergic neurons only",
"value_source_sentence": "utilizing 611,423 single-cell transcriptomes from the comprehensive and high-resolution transcriptomic and spatial cell type atlas",
"experimental_conditions": "scRNA-seq extracted from whole brain atlas"
}
],
"comparison_id": "cell-type-atlas-scale-comparison",
"comparison_name": "Scale of cell type atlases: number of cells profiled and types identified across major PV-relevant studies",
"comparison_type": "timeline",
"what_it_reveals": "The rapid scaling of single-cell transcriptomic atlases from thousands to millions of cells, with each generation revealing finer-grained PV interneuron subtypes. Earlier studies (2020) found 28 met-types from 4,200 cells; the latest (2023-2024) resolve >5,000 clusters from millions of cells.",
"homogeneity_check": {
"caveats": [
"Studies cover different brain regions: visual cortex only vs isocortex vs whole brain",
"Different studies profile all cells vs only GABAergic cells",
"Taxonomic resolution varies from 28 met-types to 5,200 clusters",
"The whole-brain atlases include non-neuronal cell types"
],
"n_definition_uniform": "true",
"scope_region_uniform": "false",
"taxonomic_level_uniform": "false",
"scope_population_uniform": "false"
},
"suggested_plot_type": "timeline"
}