Coupled-cell counts per injected astrocyte across studies/regions

{
  "papers": [
    {
      "n": 11,
      "doi": "10.1016/j.isci.2024.111043",
      "value": "30",
      "method": "intracellular dye injection (biocytin/LY/SR101) + imaging",
      "metric": "Coupled-cell count (dye-coupled cells per injection)",
      "n_analyzed": "",
      "ci_or_error": "",
      "text_access": "fulltext",
      "n_definition": "injected astrocytes",
      "scope_region": "barrel",
      "study_system": "acute CNS slice, astrocyte syncytium",
      "taxonomic_level": "per-injection cell count",
      "scope_population": "astrocytes (plus some OLs in panglial papers)",
      "value_source_sentence": "In comparison to panglial coupling in barreloids of C57BL6/J mice the number of coupled cells in Cx30 knockout mice under control conditions was reduced, because Cx30 is the main astrocytic connexin in the thalamus and dominate number of cell contacts by gap junctions to neighboring astrocytes and oligodendrocytes.",
      "experimental_conditions": "region=barrel"
    },
    {
      "n": 10,
      "doi": "10.1083/jcb.149.7.1513",
      "value": "11",
      "method": "intracellular dye injection + imaging",
      "metric": "Coupled-cell count per injected astrocyte (mean ± SEM)",
      "n_analyzed": "",
      "ci_or_error": "",
      "text_access": "fulltext",
      "n_definition": "injected astrocytes",
      "scope_region": "unspecified",
      "study_system": "acute CNS slice, astrocyte syncytium",
      "taxonomic_level": "per-injection cell count",
      "scope_population": "astrocytes",
      "value_source_sentence": "In these experiments, the number of coupled cells was increased from 11 ± 2 ( n = 36) in astrocytes cultures to 26 ± 4 ( n = 30) in N/A cocultures.",
      "experimental_conditions": "region=unspecified"
    },
    {
      "n": 6,
      "doi": "10.3389/fncel.2018.00431",
      "value": "100",
      "method": "intracellular dye injection + imaging",
      "metric": "Coupled-cell count per injected astrocyte (mean ± SEM)",
      "n_analyzed": "",
      "ci_or_error": "",
      "text_access": "fulltext",
      "n_definition": "injected astrocytes",
      "scope_region": "unspecified",
      "study_system": "acute CNS slice, astrocyte syncytium",
      "taxonomic_level": "per-injection cell count",
      "scope_population": "astrocytes",
      "value_source_sentence": "Coupling networks that were filled for at least 30 min—when increase of network size per time interval was less eminent—consisted of 100 ± 12 cells that occupied an area of 0.067 ± 0.009 mm 2 ( n = 32/32/31; not shown).",
      "experimental_conditions": "region=unspecified"
    },
    {
      "n": 56,
      "doi": "10.3390/biom12121812",
      "value": "36.9",
      "method": "intracellular dye injection + imaging",
      "metric": "Coupled-cell count per injected astrocyte (mean ± SEM)",
      "n_analyzed": "",
      "ci_or_error": "",
      "text_access": "fulltext",
      "n_definition": "injected astrocytes",
      "scope_region": "unspecified",
      "study_system": "acute CNS slice, astrocyte syncytium",
      "taxonomic_level": "per-injection cell count",
      "scope_population": "astrocytes",
      "value_source_sentence": "Alexa 568 spread to a greater number of coupled cells (36.9 ± 4.6 cells; Vm = −80 ± 0.8 mV, N = 10) and sulforhodamine B showed much more propagation with 60.4 ± 2.9 coupled cells (Vm = −79.4 ± 1.1 mV, N = 7).",
      "experimental_conditions": "region=unspecified"
    }
  ],
  "comparison_id": "coupled_cell_counts_by_region",
  "comparison_name": "Coupled-cell counts per injected astrocyte across studies/regions",
  "comparison_type": "convergent evidence",
  "what_it_reveals": "Across CNS regions and studies, single-astrocyte biocytin/LY/SR101 injections reveal networks of ~30–200 coupled cells; regional and method differences explain a substantial fraction of the range.",
  "homogeneity_check": {
    "caveats": [
      "Different dyes (biocytin, LY, Alexa488, SR-B) differ in molecular weight and gap-junction permeability.",
      "Some entries are astrocyte-only networks; others include oligodendrocytes (panglial).",
      "Culture vs acute slice differences confound comparison (10.1083/jcb.149.7.1513 is in culture)."
    ],
    "n_definition_uniform": "true",
    "scope_region_uniform": "false",
    "taxonomic_level_uniform": "true",
    "scope_population_uniform": "false"
  },
  "suggested_plot_type": "grouped bar"
}

[
  "paper:paper-0f6f851a1966",
  "paper:paper-29515899cf63",
  "paper:paper-dfd928fac4a2",
  "paper:paper-f9ce5c1173cc"
]

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  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
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  "source_commit_sha": "1a55da0634a3bc04e5688792ed12141ce271d28e",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewAstrocytes"
}