{
"papers": [
{
"n": 3579,
"doi": "10.1016/j.cell.2019.09.023",
"value": "3579",
"method": "BARseq (in situ sequencing of RNA barcodes)",
"metric": "n_neurons_projection_mapped",
"n_analyzed": "3579",
"ci_or_error": "",
"text_access": "abstract_only",
"n_definition": "single neurons with barcodes successfully recovered",
"scope_region": "single cortical area + 11 targets",
"study_system": "mouse primary auditory cortex",
"taxonomic_level": "subcategory (IT/PT/CT)",
"scope_population": "all barcode-recovered neurons",
"value_source_sentence": "Mapping the projections to 11 areas of 3,579 neurons in mouse auditory cortex using BARseq confirmed the laminar organization of the three top classes (intratelencephalic [IT], pyramidal tract-like [PT-like], and corticothalamic [CT]) of projection neurons.",
"experimental_conditions": "BARseq RNA-barcoding + 11 dissected target areas"
},
{
"n": 8000,
"doi": "10.1038/s41467-024-52756-x",
"value": "8000",
"method": "axonal BARseq",
"metric": "n_neurons_projection_mapped",
"n_analyzed": "8000",
"ci_or_error": ">8000",
"text_access": "abstract_only",
"n_definition": "single neurons with axonal barcodes recovered",
"scope_region": "single cortical area, single mouse",
"study_system": "mouse primary auditory cortex (single male)",
"taxonomic_level": "subcategory (IT/PT-like/CT)",
"scope_population": "all axonal-barcode-recovered neurons",
"value_source_sentence": "As a proof of principle, we have mapped the long-range projections of >8000 primary auditory cortex neurons from a single male mouse.",
"experimental_conditions": "axonal BARseq in single mouse"
},
{
"n": 35742,
"doi": "10.1016/j.cell.2022.09.038",
"value": "35742",
"method": "BARseq",
"metric": "n_neurons_projection_mapped",
"n_analyzed": "35742",
"ci_or_error": "",
"text_access": "abstract_only",
"n_definition": "single neurons with barcodes recovered",
"scope_region": "olfactory bulb + piriform cortex",
"study_system": "mouse olfactory bulb (5309) + piriform cortex (30433)",
"taxonomic_level": "subcategory",
"scope_population": "all barcode-recovered output neurons",
"value_source_sentence": "Here, we used high-throughput sequencing-based neuroanatomical techniques to analyze the projections of 5,309 mouse olfactory bulb and 30,433 piriform cortex output neurons at single-cell resolution.",
"experimental_conditions": "BARseq-based projection mapping"
},
{
"n": 1023,
"doi": "10.34133/research.0470",
"value": "1023",
"method": "fMOST single-neuron reconstruction",
"metric": "n_neurons_fully_reconstructed",
"n_analyzed": "1023",
"ci_or_error": "",
"text_access": "abstract_only",
"n_definition": "fully reconstructed single pyramidal neurons (fMOST)",
"scope_region": "sensorimotor cortex, 6 subregions",
"study_system": "mouse sensorimotor cortex, IT and PT Cre lines",
"taxonomic_level": "subcategory",
"scope_population": "Cre-targeted IT and PT pyramidal cells",
"value_source_sentence": "…we reconstructed the complete morphology of 1,023 pyramidal neurons and generated a projectome of 6 subregions within the sensorimotor cortex.",
"experimental_conditions": "fMOST whole-brain imaging + sparse labeling + reconstruction"
},
{
"n": 1741,
"doi": "10.1038/s41586-021-03941-1",
"value": "1741",
"method": "whole-brain single-neuron reconstruction",
"metric": "n_neurons_fully_reconstructed",
"n_analyzed": "1741",
"ci_or_error": "",
"text_access": "abstract_only",
"n_definition": "fully reconstructed single neurons",
"scope_region": "multi-region (cortex+claustrum+thalamus+striatum)",
"study_system": "mouse cortex, claustrum, thalamus, striatum",
"taxonomic_level": "subcategory",
"scope_population": "sparsely labeled neurons across multiple Cre lines",
"value_source_sentence": "We fully reconstructed 1,741 neurons from cortex, claustrum, thalamus, striatum and other brain regions in mice.",
"experimental_conditions": "sparse labeling + whole-brain imaging + reconstruction"
}
],
"audit_issues": [
{
"dimension": "scope_region",
"description": "Rows span auditory cortex, olfactory bulb + piriform cortex, sensorimotor cortex, and multi-region (cortex+claustrum+thalamus+striatum). Counts mix anatomical denominators.",
"entries_affected": [
"10.1016/j.cell.2019.09.023",
"10.1038/s41467-024-52756-x",
"10.1016/j.cell.2022.09.038",
"10.34133/research.0470",
"10.1038/s41586-021-03941-1"
]
},
{
"dimension": "study_system",
"description": "Two distinct measurement modalities are pooled: BARseq RNA-barcoded mapping (rows 1–3) and fMOST / whole-brain single-neuron reconstruction (rows 4–5). Throughput and 'fully reconstructed neuron' definitions are different.",
"entries_affected": [
"10.1016/j.cell.2019.09.023",
"10.1038/s41467-024-52756-x",
"10.1016/j.cell.2022.09.038",
"10.34133/research.0470",
"10.1038/s41586-021-03941-1"
]
},
{
"dimension": "metric_definition",
"description": "'n_neurons_projection_mapped' (BARseq, barcode recovery) and 'n_neurons_fully_reconstructed' (fMOST, dense morphology) are not the same quantity.",
"entries_affected": [
"10.1016/j.cell.2019.09.023",
"10.1038/s41467-024-52756-x",
"10.1016/j.cell.2022.09.038",
"10.34133/research.0470",
"10.1038/s41586-021-03941-1"
]
}
],
"audit_verdict": "CAVEAT",
"comparison_id": "single-neuron-projection-counts-mouse-cortex",
"comparison_name": "Single-neuron projection mapping throughput in mouse cortex",
"comparison_type": "convergent evidence",
"what_it_reveals": "BARseq and fMOST reconstructions in mouse cortex now collectively report on tens of thousands of single-neuron projections, exceeding the throughput at which earlier paired-recording-based wiring rules were established.",
"homogeneity_check": {
"caveats": [
"n_neurons compares barcode-based projection mapping (BARseq) with fMOST single-neuron reconstruction — different measurement modalities.",
"Brain regions differ (auditory cortex vs olfactory cortex vs sensorimotor cortex vs multi-region)."
],
"n_definition_uniform": "false",
"scope_region_uniform": "false",
"taxonomic_level_uniform": "true",
"scope_population_uniform": "false"
},
"suggested_plot_type": "grouped bar",
"mandatory_caption_caveats": [
"Counts mix BARseq barcode-mapped neurons (throughput-optimised, projection-target resolution) with fMOST fully-reconstructed neurons (dense single-axon morphology). The two measurements are not equivalent.",
"Cortical regions differ across rows; the figure shows method throughput across studies rather than a like-for-like cellular census."
]
}