{
"title": "Sensitivity and temporal resolution of genetically encoded neurotransmitter sensors",
"papers": [
{
"doi": "10.3390/ijms21218048",
"value": "Understanding how dopamine (DA) encodes behavior depends on technologies that can reliably monitor D",
"condition": "GPCR-Based Dopamine Sensors—A Detailed Guide to Inform Sensor Choice for In Vivo Imaging",
"value_source_sentence": "Understanding how dopamine (DA) encodes behavior depends on technologies that can reliably monitor DA release in freely-behaving animals."
},
{
"doi": "10.1093/nsr/nwae112",
"value": "660 %",
"condition": "An octopamine-specific GRAB sensor reveals a monoamine relay circuitry that boosts aversive learning",
"value_source_sentence": "We found that this sensor is highly specific to OA, has sub-second kinetics and exhibits a peak increase in fluorescence of ∼660% in response to OA."
},
{
"doi": "10.1101/2024.03.09.584200",
"value": "<h4>ABSTRACT</h4> Octopamine (OA), analogous to norepinephrine in vertebrates, is an essential monoa",
"condition": "An octopamine-specific GRAB sensor reveals a monoamine relay circuitry that boosts aversive learning",
"value_source_sentence": "<h4>ABSTRACT</h4> Octopamine (OA), analogous to norepinephrine in vertebrates, is an essential monoamine neurotransmitter in invertebrates that plays a significant role in various biological functions, including olfactory associative learning."
},
{
"doi": "10.1254/fpj.24111",
"value": "However, achievement of high spatial and temporal resolution in neuromodulator measurements has pres",
"condition": "[Real-time measurement of neuromodulators using GRAB sensors].",
"value_source_sentence": "However, achievement of high spatial and temporal resolution in neuromodulator measurements has presented significant challenges, particularly in the context of real-time observations within freely behaving animals."
},
{
"doi": "10.1101/332528",
"value": "ΔF/F = 0%",
"condition": "A genetically-encoded fluorescent sensor enables rapid and specific detection of dopamine in flies, ",
"value_source_sentence": "ΔF/F 0 "
},
{
"doi": "10.1038/s41467-023-41581-3",
"value": "10 Hz",
"condition": "Distinct sub-second dopamine signaling in dorsolateral striatum measured by a genetically-encoded fl",
"value_source_sentence": "Fast-scan cyclic voltammetry is an electrochemical method that can be used to measure phasic release of catecholamines at sampling rates of 10 Hz or higher 8 ."
}
],
"x_axis": "Sensor",
"y_axis": "Affinity (EC50, nM) and Response Time (ms)",
"n_analyzed": "N/A - sensor characterization",
"n_definition": "sensor variant characterized",
"scope_region": "in vitro characterization and in vivo validation",
"comparison_id": "sensor_sensitivity_comparison",
"comparison_type": "cross-study comparison of sensor characteristics",
"taxonomic_level": "N/A",
"scope_population": "genetically encoded GPCR-based sensors",
"homogeneity_check": {
"caveats": [
"EC50 values measured under different conditions across labs",
"Response times depend on expression level and recording geometry",
"In vitro affinity may not match in vivo sensitivity",
"Different studies use different metrics (EC50, Kd, ΔF/F max)"
],
"comparable": "partially"
}
}