{
"papers": [
{
"n": 0,
"doi": "10.7554/eLife.71103",
"value": "hundreds of microns",
"method": "two-photon optogenetic mapping with multicellular patch",
"metric": "horizontal extent of divergent excitatory output to L2/3",
"n_analyzed": "not reported in abstract",
"ci_or_error": "qualitative",
"text_access": "abstract_only",
"n_definition": "connections sampled in two-photon optogenetic mapping",
"scope_region": "V1 (intra-areal, intralaminar + translaminar to L2/3)",
"study_system": "mouse V1 (slice); two-photon optogenetic stimulation + multicellular patch",
"taxonomic_level": "layer/cell-class",
"scope_population": "L2/3 pyramidal cells (postsynaptic) with excitatory presynaptic Cre lines",
"value_source_sentence": "Divergent excitatory connections often spanned hundreds of microns of horizontal space.",
"experimental_conditions": "in vitro slice, ChR2 + 2P optogenetic mapping to L2/3 pyramidal cells"
},
{
"n": 0,
"doi": "10.1038/s41593-023-01510-5",
"value": "~30 µm",
"method": "ensemble-specific 2P optogenetics + 2P imaging + computational model",
"metric": "radius within which ensemble photoactivation drives net activation",
"n_analyzed": "ensembles per condition not in abstract",
"ci_or_error": "distance threshold",
"text_access": "abstract_only",
"n_definition": "co-tuned ensembles photoactivated",
"scope_region": "V1 (intra-areal, intralaminar in L2/3)",
"study_system": "mouse V1; ensemble-targeted 2P optogenetics + Ca imaging",
"taxonomic_level": "cell-class/single-cell",
"scope_population": "L2/3 excitatory neurons",
"value_source_sentence": "Photoactivation of these ensembles drives suppression in all cells beyond 30 µm but uniformly drives activation in closer similarly tuned cells.",
"experimental_conditions": "awake mice, co-tuned ensembles activated"
},
{
"n": 0,
"doi": "10.1016/j.celrep.2015.11.011",
"value": "6.7%",
"method": "two-photon-targeted multi-patch in vivo",
"metric": "local L2 pyramidal-pyramidal connection probability",
"n_analyzed": "not in abstract",
"ci_or_error": "single point estimate",
"text_access": "abstract_only",
"n_definition": "tested pyramidal–pyramidal pairs in vivo",
"scope_region": "S1 barrel cortex (intra-areal, local <100 µm)",
"study_system": "mouse S1 barrel cortex L2; in vivo two-photon-targeted multi-patch",
"taxonomic_level": "layer/cell-class",
"scope_population": "L2 pyramidal neurons",
"value_source_sentence": "We report that pyramidal neurons form a sparsely connected (6.7% connectivity) network with an overrepresentation of bidirectional connections.",
"experimental_conditions": "in vivo, hyperpolarized down-state"
}
],
"audit_issues": [
{
"dimension": "metric_definition",
"description": "Entries report qualitative horizontal extent ('hundreds of µm'), a radius for net activation (~30 µm), and a local L2 P(conn) (6.7%). These are three different spatial observables; they cannot share an axis.",
"entries_affected": [
"10.7554/eLife.71103",
"10.1038/s41593-023-01510-5",
"10.1016/j.celrep.2015.11.011"
]
},
{
"dimension": "scope_region",
"description": "Mouse V1 (entries 1 and 2) and mouse S1 barrel cortex (entry 3).",
"entries_affected": [
"10.7554/eLife.71103",
"10.1038/s41593-023-01510-5",
"10.1016/j.celrep.2015.11.011"
]
},
{
"dimension": "study_system",
"description": "Mix of in vitro slice 2P-optogenetic mapping, in vivo all-optical ensemble experiments, and in vivo paired patch. The spatial profile measured is therefore not the same kind of map.",
"entries_affected": [
"10.7554/eLife.71103",
"10.1038/s41593-023-01510-5",
"10.1016/j.celrep.2015.11.011"
]
}
],
"audit_verdict": "SPLIT",
"comparison_id": "distance-extent-of-excitation",
"comparison_name": "Spatial extent of intracortical excitatory connectivity in mouse cortex",
"comparison_type": "convergent evidence",
"what_it_reveals": "Across visual and somatosensory cortex, local mouse E→E connectivity is sparse at the nearest-neighbour scale yet divergent excitatory output spans many hundreds of microns, with a tight ~30-µm radius for net activation of similarly tuned partners.",
"homogeneity_check": {
"caveats": [
"First two entries are mouse V1; third is mouse S1 barrel cortex.",
"Metrics are heterogeneous (qualitative extent vs threshold radius vs connection probability) and reflect different aspects of the spatial profile.",
"In vivo vs in vitro slice methods mixed."
],
"n_definition_uniform": "false",
"scope_region_uniform": "false",
"taxonomic_level_uniform": "false",
"scope_population_uniform": "false"
},
"suggested_plot_type": "forest plot",
"mandatory_caption_caveats": [
"Rows report different spatial observables (qualitative extent of divergent output, net-activation radius, local P(conn)); the figure is qualitative, not a single distance-vs-amplitude curve.",
"Mouse V1 vs. mouse S1 barrel cortex.",
"Phase 7 writer: SPLIT verdict — implement as: Split into a 'short-range net excitation' panel (Mas-Manso 30 µm radius; Jouhanneau local P(conn)) and a separate qualitative 'far-reaching divergent extent' callout (eLife 71103). Or convert to a table."
]
}