Version history

{
  "kind": "infographic",
  "prompt": "A hierarchical transcription factor cascade (NKX2.1→LHX6→ARX/CXCR7/SATB1) controls PV interneuron specification, with each factor regulating distinct aspects (regional identity, fate determination, laminar positioning, maturation)",
  "provider": "other",
  "raw_fields": {
    "papers": [
      {
        "n": 0,
        "doi": "10.1242/dev.015123",
        "value": "NKX2.1 binds conserved sequence in Lhx6 promoter for direct activation",
        "method": "promoter binding assay, transplantation fate analysis",
        "metric": "NKX2.1 → Lhx6 direct activation",
        "n_analyzed": null,
        "ci_or_error": null,
        "text_access": "abstract_only",
        "n_definition": "experiments",
        "scope_region": "MGE/cortex",
        "study_system": "mouse, Nkx2.1-/-, slice culture transplant",
        "taxonomic_level": "MGE-derived interneurons",
        "scope_population": "MGE progenitors",
        "value_source_sentence": "NKX2.1 protein binds a highly conserved sequence in the Lhx6 promoter, and this sequence appears to mediate the direct activation of Lhx6 by NKX2.1.",
        "experimental_conditions": "electroporation of Nkx2.1 cDNA"
      },
      {
        "n": 0,
        "doi": "10.1016/j.neuron.2014.02.030",
        "value": "Lhx6 directly binds Arx enhancer and CXCR7 intronic enhancer",
        "method": "ChIP, in vivo binding, fate analysis",
        "metric": "Lhx6 → Arx/CXCR7 regulation",
        "n_analyzed": null,
        "ci_or_error": null,
        "text_access": "fulltext",
        "n_definition": "experiments",
        "scope_region": "MGE/cortex",
        "study_system": "mouse, Lhx6-/-, MGE transplantation",
        "taxonomic_level": "MGE-derived interneurons",
        "scope_population": "MGE-derived interneurons",
        "value_source_sentence": "Lhx6 directly binds in vivo to an Arx enhancer and to an intronic CXCR7 enhancer that remains active in mature interneurons.",
        "experimental_conditions": "complementation/transplantation assay"
      },
      {
        "n": 0,
        "doi": "10.1016/j.celrep.2012.10.003",
        "value": "SATB1 required for PV expression; loss causes dramatic PV+ decrease",
        "method": "immunohistochemistry, fate mapping",
        "metric": "SATB1 as PV maturation effector downstream of Lhx6",
        "n_analyzed": null,
        "ci_or_error": null,
        "text_access": "fulltext",
        "n_definition": "animals per genotype",
        "scope_region": "cortex",
        "study_system": "mouse, Satb1 conditional knockout",
        "taxonomic_level": "PV+ interneurons specifically",
        "scope_population": "PV-positive interneurons",
        "value_source_sentence": "Satb1 mutant cortex showed a dramatic decrease in the number of cells expressing PV, while the number of SST-expressing cells was largely unaffected.",
        "experimental_conditions": "MGE-specific Satb1 deletion"
      }
    ],
    "comparison_id": "transcription-factor-cascade-mge-pv-specification",
    "comparison_name": "Transcription Factor Cascade for MGE-Derived PV Interneuron Specification",
    "comparison_type": "convergent evidence",
    "what_it_reveals": "A hierarchical transcription factor cascade (NKX2.1→LHX6→ARX/CXCR7/SATB1) controls PV interneuron specification, with each factor regulating distinct aspects (regional identity, fate determination, laminar positioning, maturation)",
    "homogeneity_check": {
      "caveats": [
        "Different stages of the cascade: specification vs fate vs maturation",
        "Different knockout models at different developmental stages",
        "SATB1 paper focuses specifically on PV+ while others examine broader MGE-derived populations"
      ],
      "n_definition_uniform": "false",
      "scope_region_uniform": "true",
      "taxonomic_level_uniform": "false",
      "scope_population_uniform": "false"
    },
    "suggested_plot_type": "timeline"
  },
  "section_id": "section_03_evidence_package",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewPV/blob/df9fc7e8d455b084152c9d713558dae0013cef21/evidence/section_03_evidence_package.json",
  "target_ref": "wiki_page:computationalreviewpv-03",
  "review_repo": "ComputationalReviewPV",
  "section_ref": "wiki_page:computationalreviewpv-03",
  "source_path": "evidence/section_03_evidence_package.json",
  "source_refs": [
    "paper:paper-09e3387a22aa",
    "paper:paper-a41ff0022587",
    "paper:paper-a9cd8f097456"
  ],
  "section_title": "Developmental Origins: MGE Lineage, Specification, and Migration",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "df9fc7e8d455b084152c9d713558dae0013cef21",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewPV"
  },
  "generation_status": "complete",
  "review_bundle_ref": "analysis_bundle:ab-e6261c8263e7",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewPV/blob/df9fc7e8d455b084152c9d713558dae0013cef21/evidence/section_03_evidence_package.json",
  "commit_sha": "df9fc7e8d455b084152c9d713558dae0013cef21",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewPV"
}