Gliotransmission: Evidence and Controversy figure 2

{
  "topic": "Effect of astrocytic dnSNARE expression on sleep homeostasis / sleep rebound / sleep-loss cognitive consequences",
  "metric": "Sleep parameters and sleep-loss behavioral/EEG readouts in dnSNARE vs wild-type littermates",
  "papers": [
    {
      "n": 17,
      "doi": "10.1016/j.neuron.2008.11.024",
      "label": "Halassa 2009 — dnSNARE mouse sleep rebound",
      "value": "TST rebound: WT 7.53 ± 1.13% (n=9) vs dnSNARE 1.89 ± 1.30% (n=8)",
      "direction": "decrease",
      "n_analyzed": 17,
      "n_definition": "n = number of mice; mixed genotype totals reported",
      "scope_region": "Whole-mouse cortical EEG / behavioral sleep scoring",
      "taxonomic_level": "whole-animal sleep-recording cohort",
      "scope_population": "Adult male mice, GFAP-dnSNARE vs WT",
      "value_source_sentence": "Astrocytic dnSNARE expression reduced the increase in total sleep time (TST) following sleep deprivation (wild-type = 7.53% ± 1.13%, n = 9 mice; dnSNARE = 1.89% ± 1.30%, n = 8 mice; unpaired t test, p   0.01) ( Figures 2 D, 2E, and S9 ).",
      "numeric_value_rebound_pct_wt": 7.53,
      "numeric_value_rebound_pct_dnSNARE": 1.89
    },
    {
      "n": 8,
      "doi": "10.1038/tp.2012.136",
      "label": "Hines 2013 — dnSNARE antidepressant (sleep-deprivation) behavioral phenotype",
      "value": "FST immobility: WT SD=28.83±5.70 s vs dnSNARE SD=161.58±16.31 s (dnSNARE unresponsive to SD)",
      "direction": "decrease_in_effect_of_SD",
      "n_analyzed": 8,
      "n_definition": "n = mice per genotype group",
      "scope_region": "Whole animal behavioral assay (FST) after sleep deprivation",
      "taxonomic_level": "whole-animal behavior cohort",
      "scope_population": "Adult GFAP-dnSNARE vs WT mice",
      "value_source_sentence": "In the forced-swim test, sleep deprivation reduced the immobility time from 140.813±6.97 to 28.83±5.70 s ( P  0.001) in wild-type mice; whereas in dnSNARE mice, basal immobility (186.99±13.44 s) was not significantly affected by sleep deprivation (161.58±16.31 s; P =0.129; n =8 per group).",
      "numeric_value_immobility_WT_SD_s": 28.83,
      "numeric_value_immobility_dnSNARE_SD_s": 161
    },
    {
      "n": 0,
      "doi": "10.1523/jneurosci.2585-14.2014",
      "label": "Fujita 2014 — dnSNARE transgene neuronal leakage confound",
      "value": "Widespread neuronal expression of dnSNARE transgene in cortical neurons; reversible suppression of neuronal activity (qualitative)",
      "direction": "confound",
      "n_analyzed": null,
      "n_definition": "Percent neurons expressing transgene (no mouse-level n in abstract)",
      "scope_region": "Mouse cortex, hippocampus, amygdala",
      "taxonomic_level": "tissue-level immunohistochemistry / reporter quantification",
      "scope_population": "GFAP-tTA::tetO-dnSNARE transgenic mice",
      "value_source_sentence": "Inconsistent with this premise, we report here widespread expression of the dnSNARE transgene in cortical neurons.",
      "numeric_value_neuronal_leak_present": true
    }
  ],
  "comparison_id": "FD2_dnSNARE_sleep_homeostasis",
  "interpretation": "The dnSNARE mouse shows robust sleep-homeostasis (reduced TST rebound) and sleep-loss cognitive phenotypes that Halassa/Hines attribute to blockade of astrocyte vesicular release. Fujita 2014 shows neuronal transgene expression in the same line, raising the possibility that the phenotype reflects partial neuronal SNARE block rather than a pure astrocytic effect.",
  "homogeneity_check": {
    "same_genotype": true,
    "comparability_caveats": "Papers measure different dependent variables (sleep rebound %, immobility time, transgene expression) but all report on the same dnSNARE mouse line and directly bear on whether the observed phenotypes are astrocyte-specific. The Fujita 2014 neuronal-leak finding is a meta-methodological confound that applies to both Halassa and Hines measurements.",
    "same_mouse_line_family": true,
    "same_dependent_variable": false
  }
}

[
  "paper:paper-5fe7a4267091",
  "paper:paper-752d0a3a1250",
  "paper:paper-eb1a53222f37"
]

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  "source_commit_sha": "1a55da0634a3bc04e5688792ed12141ce271d28e",
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