Version history

{
  "kind": "infographic",
  "prompt": "Astrocyte Identity and Diversity figure 1",
  "provider": "other",
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    "name": "astrocyte_subtype_counts_across_studies",
    "metric": "number of astrocyte subtypes identified",
    "papers": [
      {
        "doi": "10.1038/s41467-019-14198-8",
        "year": "2020",
        "title": "Identification of region-specific astrocyte subtypes at single cell resolution.",
        "value": 5,
        "metric": "number of astrocyte subtypes/clusters identified",
        "study_system": "mouse (scRNA-seq)",
        "value_source_sentence": "This led to the identification of five distinct Astrocyte SubTypes (AST1–5), each distinguished by a gene expression fingerprint (Fig."
      },
      {
        "doi": "10.1038/s41467-025-63429-8",
        "year": "2025",
        "title": "Aging in mice alters regionally enriched striatal astrocytes.",
        "value": 7,
        "metric": "number of astrocyte subtypes/clusters identified",
        "study_system": "mouse (scRNA-seq)",
        "value_source_sentence": "1 ), power analysis to assess if our data have enough cells to identify 7 astrocyte subtypes in the striatum (Supplementary Fig."
      },
      {
        "doi": "10.1038/s41467-025-61829-4",
        "year": "2025",
        "title": "Dual lineage origins contribute to neocortical astrocyte diversity.",
        "value": 5,
        "metric": "number of astrocyte subtypes/clusters identified",
        "study_system": "mouse (scRNA-seq, spatial transcriptomics)",
        "value_source_sentence": "These methods allowed us to identify five molecularly distinct astrocyte subtypes in the mouse neocortex, each with unique localizations along the cortical column and distinct predicted functions."
      },
      {
        "doi": "10.1038/s41398-025-03616-9",
        "year": "2025",
        "title": "Developmental arrest of astrocyte lineage in Snai2 deletion mice: implication for the inte",
        "value": 5,
        "metric": "number of astrocyte subtypes/clusters identified",
        "study_system": "human+mouse (spatial transcriptomics)",
        "value_source_sentence": "Moreover, five astrocyte clusters were identified in brains of Snai2 + / + and Snai2 − / − mice according to their transcriptomic signatures (Table S2 )."
      },
      {
        "doi": "10.1038/s41593-020-0602-1",
        "year": "2020",
        "title": "Astrocyte layers in the mammalian cerebral cortex revealed by a single-cell in situ transc",
        "value": 2,
        "metric": "number of astrocyte subtypes/clusters identified",
        "study_system": "human+mouse (scRNA-seq, spatial transcriptomics)",
        "value_source_sentence": "Unsupervised clustering with the Louvain method identified two major astrocyte subtypes in the P56 cortex ( Fig 4C )."
      },
      {
        "doi": "10.1038/s41593-024-01743-y",
        "year": "2024",
        "title": "Characteristics of blood-brain barrier heterogeneity between brain regions revealed by pro",
        "value": 4,
        "metric": "number of astrocyte subtypes/clusters identified",
        "study_system": "mouse (scRNA-seq, spatial transcriptomics, EM)",
        "value_source_sentence": "Subclustering analysis identified four astrocyte subtypes, one from the cortex and three from the ME (Fig."
      }
    ],
    "x_axis": "study (first author, year)",
    "y_axis": "number of subtypes",
    "description": "Bar chart comparing the number of astrocyte subtypes or clusters identified across recent single-cell / spatial transcriptomic studies of rodent and human brain. Values range from 2 to 7 depending on tissue scope, cell numbers, clustering resolution, and whether layer-dependent versus unsupervised clustering is used.",
    "figure_type": "bar",
    "homogeneity_check": {
      "caveats": "Studies span different brain regions (forebrain in Batiuk 2020; cortex-only in Bayraktar 2020 and Cuevas-Diaz Duran 2025; cortex+median eminence in Chen 2024; striatum in Lee 2025). Resolution parameters and clustering algorithms differ (Louvain, Seurat SNN, Leiden), which directly affects cluster count. Bayraktar 2020 reports 2 by unsupervised clustering but 3 gradient-based layer identities; values here take the unsupervised clustering result as reported. The Snai2 study (Dai 2025) reports 5 clusters in control mice. Studies with >=5 subtypes generally used tissue dissociation followed by deep 10x chromium sequencing, while 2-subtype studies used lower-depth smart-seq2 or in situ transcriptomics.",
      "metric_uniform": true,
      "scope_region_uniform": false,
      "taxonomic_level_uniform": false,
      "scope_population_uniform": false
    }
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  "section_title": "Astrocyte Identity and Diversity",
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