Version history

{
  "kind": "infographic",
  "prompt": "Astrocyte Calcium Signaling figure 2",
  "provider": "other",
  "raw_fields": {
    "papers": [
      {
        "doi": "10.1016/j.ceca.2013.09.001",
        "note": "Ding 2013: LC-NE ablation (DSP-4) and α1-AR blockade (prazosin) both abolish awake-mouse astrocyte Ca2+",
        "value": ">90% NE depletion (DSP-4) nearly eliminates astrocyte Ca2+; prazosin suppresses spontaneous + evoked signals",
        "intervention": "LC lesion (DSP-4) + α1-AR blockade (prazosin)",
        "measurements": [
          {
            "value": ">90% NE depletion; prevented nearly all astrocyte Ca2+",
            "intervention": "DSP-4 (LC noradrenergic lesion)",
            "value_source_sentence": "The LC-specific neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4), which reduced cortical NE content by >90%, prevented nearly all astrocytic Ca2+ signals in awake mice."
          },
          {
            "value": "suppressed spontaneous and evoked Ca2+",
            "intervention": "prazosin (α1-AR antagonist)",
            "value_source_sentence": "However, this study shows that spontaneous Ca 2+ increases are also potently inhibited by the α 1 -receptor antagonist prazosin, suggesting that the majority of astrocytic Ca 2+ increases in awake mice require α 1 -receptor activation."
          }
        ],
        "value_source_sentence": "The LC-specific neurotoxin N-(2-chloroethyl)-N-ethyl-2-bromobenzylamine (DSP-4), which reduced cortical NE content by >90%, prevented nearly all astrocytic Ca2+ signals in awake mice."
      },
      {
        "doi": "10.1523/jneurosci.5289-11.2011",
        "note": "Takata 2011: cholinergic plasticity via astrocyte Ca2+ is IP3R2-dependent",
        "value": "astrocyte [Ca2+] surges diminished; plasticity lost",
        "intervention": "IP3R2-KO + cholinergic (mAChR) stimulation",
        "value_source_sentence": "During the induction of this synaptic plasticity, we find that astrocytic [Ca(2+)](i) is pronouncedly elevated, which is blocked by mAChR antagonists."
      },
      {
        "doi": "10.1073/pnas.1209448109",
        "note": "Thrane 2012: arousal-dependent astrocyte Ca2+ in cortex",
        "value": "anesthesia selectively disrupts astrocyte Ca2+",
        "intervention": "general anesthesia vs awake",
        "value_source_sentence": "In our study, we performed calcium imaging in awake head-restrained mice and found that three commonly used anesthetic combinations (ketamine/xylazine, isoflurane, and urethane) markedly suppressed calcium transients in neocortical astrocytes."
      }
    ],
    "axis_label": "Intervention effect on cortical astrocyte Ca2+ signals",
    "comparison_topic": "Sources of astrocyte Ca2+ signals in awake cortex: noradrenergic (alpha1-AR/LC) vs cholinergic (muscarinic/NBM) pathways"
  },
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  "review_repo": "ComputationalReviewAstrocytes",
  "section_ref": "wiki_page:computationalreviewastrocytes-03",
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  "source_refs": [
    "paper:paper-47d971dd17dc",
    "paper:paper-5e6adeb1c300",
    "paper:paper-92ab47dc5417"
  ],
  "section_title": "Astrocyte Calcium Signaling",
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}