{
"axes": {
"x": "source layer (L2/3, L5, L6)",
"y": "fraction of retrogradely labeled cortico-cortical neurons"
},
"datasets": [
{
"doi": "10.7554/elife.100478",
"method": "retro-AAV from each target area, layer-resolved counting, NTSR1-Cre to subtract L6 CT",
"system": "mouse VISp, SSp-bfd, MOp",
"value_summary": "L6 (corticocortical, non-NTSR1) dominates the majority of source areas, followed by L5; L2/3 dominates only a small fraction.",
"claim_source_sentence": "When pooled across target areas, we find that the majority of projection source areas display L6 dominance, followed by L5 with only a small fraction of areas exhibiting L2/3 dominance (, p < 0.05, one-way ANOVA, Tukey–Kramer multiple comparison correction)."
},
{
"doi": "10.1002/cne.70111",
"method": "projection- and layer-specific rabies tracing onto FF and FB cortico-cortical neurons in L2/3 and L5",
"system": "mouse VISl (secondary visual cortex)",
"value_summary": "Both FF and FB types receive majority input from VISp; proportions of inputs from specific visual, retrosplenial and auditory cortices differ between FF and FB.",
"claim_source_sentence": "Overall, long-distance input patterns for these FF and FB neurons were largely similar, as all received the majority of their inputs from VISp."
},
{
"doi": "10.1016/j.neuron.2025.10.019",
"method": "single-axon reconstruction of ~20,000 cortical neurons",
"system": "whole mouse cortex (single-neuron projectomes)",
"value_summary": "346 projection-defined subtypes with region- and layer-specific distributions; seven cortico-cortical modules with submodular organization.",
"claim_source_sentence": "Here, we reconstructed the projectomes of nearly 20,000 neurons in the whole mouse cortex, identifying 346 projection-defined subtypes with region- and layer-specific distributions."
}
],
"audit_issues": [
{
"dimension": "metric_definition",
"description": "Studies report 'fraction of retrogradely labelled cells by layer' (eLife 100478), 'majority input source areas' (rabies, J Comp Neurol 70111), and 'projection-defined subtype counts across cortex' (Yan/Neuron 2025). The first is a fraction by source layer; the second a fraction by source area; the third a subtype catalogue. They cannot share a y-axis of 'fraction of source-layer cells'.",
"entries_affected": [
"10.7554/elife.100478",
"10.1002/cne.70111",
"10.1016/j.neuron.2025.10.019"
]
},
{
"dimension": "scope_population",
"description": "Retrograde from target area (counts source neurons) vs. monosynaptic rabies from a defined postsynaptic class (counts presynaptic input neurons) vs. single-neuron projectome reconstruction (counts whole-axon morphologies). Population denominators differ.",
"entries_affected": [
"10.7554/elife.100478",
"10.1002/cne.70111",
"10.1016/j.neuron.2025.10.019"
]
}
],
"audit_verdict": "CAVEAT",
"figure_concept": "Layer of origin for cortico-cortical input onto primary sensory and motor cortex (mouse): infragranular dominance",
"interpretation_note": "Across multiple recent mouse studies, the dominant source layer of long-range cortico-cortical input is not L2/3 (supragranular) but the infragranular L5/L6 compartment — refining the textbook supragranular-feedforward / infragranular-feedback dichotomy.",
"mandatory_caption_caveats": [
"Rows use different denominators (cell-counts in target-injected retrograde vs. rabies-traced presynaptic cells onto a defined class vs. whole-axon morphologies); 'fraction by source layer' is not strictly defined the same way across rows."
]
}