A de novo protein binder targeting the CD38 catalytic cleft (hotspot residues C119, K121, W125, F143, E146, D155, L157, Q226 from PDB 2I65, 1.9 Å resolution) is hypothesized to competitively inhibit CD38 NADase/cyclase activity and elevate tissue NAD+ levels in aged tissues, counteracting the NAD+ decline hallmark of aging.

Details

local_id
claim-cd38-nadsase-inhibition-hypothesis
confidence
moderate
created_by
persona-kris-ganjam
Raw fields (3)
tags
[
  "CD38",
  "NAD+",
  "senescence",
  "anti-aging",
  "BindCraft",
  "catalytic-inhibition"
]
links
{
  "source_papers": [],
  "source_datasets": [
    "PDB:2I65",
    "UniProt:P28907"
  ],
  "supporting_figures": []
}
analysis
CD38 (UniProt P28907) is the dominant NAD+ consumer in aged tissues. Catalytic cleft hotspot residues C119, K121, D155, D156, L157, D179, C201 were identified from PDB 2I65 (apo, 1.9 Å) and PDB 4XJS (inhibitor-bound, 2.8 Å, quinoline carboxamide). Centroid coordinates locked for BindCraft conditioning: C119(-10.921, 6.311, -9.356), K121(-14.362, 5.x, x.x). Identification strategy: in_silico_KO.

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