Lhx6-GFP-negative cells dissected from CGE/LGE produce diverse Vip-, Id2-, and…

Lhx6-GFP-negative cells dissected from CGE/LGE produce diverse Vip-, Id2-, and Sncg-aligned postmitotic precursor states distinct from Lhx6-GFP-positive MGE-derived cells, allowing dissection of the CGE-specific developmental trajectory; FACS-purification with stringent Lhx6+ filtering (≥20% Lhx6 mRNA threshold) confirms the CGE specificity.

{
  "n": null,
  "doi": "10.1038/nature25999",
  "claim": "Lhx6-GFP-negative cells dissected from CGE/LGE produce diverse Vip-, Id2-, and Sncg-aligned postmitotic precursor states distinct from Lhx6-GFP-positive MGE-derived cells, allowing dissection of the CGE-specific developmental trajectory; FACS-purification with stringent Lhx6+ filtering (≥20% Lhx6 mRNA threshold) confirms the CGE specificity.",
  "title": null,
  "cite_key": "Mayer2018",
  "evidence": "Lhx6-GFP transgenic mouse line, FACS-purified GFP-positive (MGE) and GFP-negative (CGE/LGE) cells; diffusion map analysis; clustering with Lhx6 contamination filtering.",
  "effect_size": "20% Lhx6 mRNA threshold for CGE-specific clusters; clear MGE/CGE postmitotic separation",
  "figure_data": [],
  "text_access": "fulltext",
  "study_system": "mouse, MGE/CGE FACS-sorted progenitors (Lhx6-GFP)",
  "_source_cluster": "cluster_02_development_lineage",
  "replication_status": "primary",
  "_source_cluster_index": 92,
  "claim_source_sentence": "H) Our Lhx6-GFP-negative dataset contains both mitotic and postmitotic cells from the CGE and diffusion map analysis shows our previously defined maturation trajectory. K) To avoid the possibility of FACS false negative MGE cells contaminating our Lhx6-GFP-negative dataset, we clustered the postmitotic cells from this dataset, and filtered out three rare clusters where Lhx6 mRNA expression was detected in more than 20% of cells ( Supplementary Methods ).",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": "K) To avoid the possibility of FACS false negative MGE cells contaminating our Lhx6-GFP-negative dataset, we clustered the postmitotic cells from this dataset, and filtered out three rare clusters where Lhx6 mRNA expression was detected in more than 20% of cells ( Supplementary Methods )."
}

[
  "paper:paper-c3e9fd7af6d3"
]

H) Our Lhx6-GFP-negative dataset contains both mitotic and postmitotic cells from the CGE and diffusion map analysis shows our previously defined maturation trajectory. K) To avoid the possibility of FACS false negative MGE cells contaminating our Lhx6-GFP-negative dataset, we clustered the postmitotic cells from this dataset, and filtered out three rare clusters where Lhx6 mRNA expression was detected in more than...

[
  {
    "ref": "paper:paper-c3e9fd7af6d3"
  }
]

{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "95e761177f7d2ec565983d3307c14ec238f9677c",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP"
}