Version history

{
  "scope": "mouse cortex in vivo, two-photon holographic optogenetics + whole-cell recording, methodological",
  "claim_text": "Combining two-photon holographic optogenetic stimulation of presynaptic candidates with whole-cell recording from a postsynaptic neuron and compressive-sensing reconstruction substantially increases the throughput of in vivo monosynaptic excitatory–excitatory connectivity mapping in mouse cortex.",
  "raw_fields": {
    "n": 0,
    "doi": "10.1038/s41593-025-02024-y",
    "claim": "Combining two-photon holographic optogenetic stimulation of presynaptic candidates with whole-cell recording from a postsynaptic neuron and compressive-sensing reconstruction substantially increases the throughput of in vivo monosynaptic excitatory–excitatory connectivity mapping in mouse cortex.",
    "cite_key": "Chen2025b",
    "evidence": "Combined in vivo two-photon holographic patterned photostimulation of candidate presynaptic pyramidal neurons with simultaneous whole-cell recording from postsynaptic targets in mouse cortex; sparse-recovery via compressive sensing applied to multi-cell stimulation patterns.",
    "effect_size": "Multi-cell holographic stim + compressive sensing recovers connectivity orders of magnitude faster than sequential single-cell probing",
    "text_access": "fulltext",
    "study_system": "mouse cortex in vivo, two-photon holographic optogenetics + whole-cell recording, methodological",
    "argument_role": "supporting",
    "replication_status": "single_study",
    "claim_source_sentence": "Here, we present a framework for increasing mapping throughput and speed that combines two-photon holographic optogenetic stimulation of presynaptic neurons, whole-cell recordings of postsynaptic responses and compressive sensing reconstruction of sparse connectivity.",
    "source_provenance_status": "ok",
    "replication_evidence_dois": [],
    "effect_size_source_sentence": "We further demonstrate the speed and throughput of the method by combining 2P optogenetic multi-cell stimulation with compressive sensing (CS), an approach that uses sparsity and incoherent sampling to find a solution to the unknown connectivity vector in an underdetermined linear system of equations."
  },
  "section_id": "section_05",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_05_evidence_package.json",
  "effect_size": "Multi-cell holographic stim + compressive sensing recovers connectivity orders of magnitude faster than sequential single-cell probing",
  "review_repo": "ComputationalReviewRecurrence",
  "section_ref": "wiki_page:computationalreviewrecurrence-05-horizontal",
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    "paper:paper-218c7dbe65b1"
  ],
  "source_span": "Here, we present a framework for increasing mapping throughput and speed that combines two-photon holographic optogenetic stimulation of presynaptic neurons, whole-cell recordings of postsynaptic responses and compressive sensing reconstruction of sparse connectivity.",
  "study_system": "mouse cortex in vivo, two-photon holographic optogenetics + whole-cell recording, methodological",
  "evidence_refs": [
    {
      "ref": "paper:paper-218c7dbe65b1"
    }
  ],
  "section_title": "5. Horizontal long-range intracortical excitatory connections in mouse — patchy L2/3-L5 axons, similarity tuning, distance-decay",
  "source_policy": {
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    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
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  "evidence_summary": "Combined in vivo two-photon holographic patterned photostimulation of candidate presynaptic pyramidal neurons with simultaneous whole-cell recording from postsynaptic targets in mouse cortex; sparse-recovery via compressive sensing applied to multi-cell stimulation patterns.",
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  "replication_status": "single_study",
  "review_package_ref": "analysis_bundle:ab-d9c479db9be9",
  "source_artifact_ref": "wiki_page:computationalreviewrecurrence-05-horizontal",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_05_evidence_package.json",
  "commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
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  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}