Combining two-photon holographic optogenetic stimulation of presynaptic candida…

Combining two-photon holographic optogenetic stimulation of presynaptic candidates with whole-cell recording from a postsynaptic neuron and compressive-sensing reconstruction substantially increases the throughput of in vivo monosynaptic excitatory–excitatory connectivity mapping in mouse cortex.

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  "doi": "10.1038/s41593-025-02024-y",
  "claim": "Combining two-photon holographic optogenetic stimulation of presynaptic candidates with whole-cell recording from a postsynaptic neuron and compressive-sensing reconstruction substantially increases the throughput of in vivo monosynaptic excitatory–excitatory connectivity mapping in mouse cortex.",
  "cite_key": "Chen2025b",
  "evidence": "Combined in vivo two-photon holographic patterned photostimulation of candidate presynaptic pyramidal neurons with simultaneous whole-cell recording from postsynaptic targets in mouse cortex; sparse-recovery via compressive sensing applied to multi-cell stimulation patterns.",
  "effect_size": "Multi-cell holographic stim + compressive sensing recovers connectivity orders of magnitude faster than sequential single-cell probing",
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  "study_system": "mouse cortex in vivo, two-photon holographic optogenetics + whole-cell recording, methodological",
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  "claim_source_sentence": "Here, we present a framework for increasing mapping throughput and speed that combines two-photon holographic optogenetic stimulation of presynaptic neurons, whole-cell recordings of postsynaptic responses and compressive sensing reconstruction of sparse connectivity.",
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  "effect_size_source_sentence": "We further demonstrate the speed and throughput of the method by combining 2P optogenetic multi-cell stimulation with compressive sensing (CS), an approach that uses sparsity and incoherent sampling to find a solution to the unknown connectivity vector in an underdetermined linear system of equations."
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Here, we present a framework for increasing mapping throughput and speed that combines two-photon holographic optogenetic stimulation of presynaptic neurons, whole-cell recordings of postsynaptic responses and compressive sensing reconstruction of sparse connectivity.

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Combined in vivo two-photon holographic patterned photostimulation of candidate presynaptic pyramidal neurons with simultaneous whole-cell recording from postsynaptic targets in mouse cortex; sparse-recovery via compressive sensing applied to multi-cell stimulation patterns.