Version history

{
  "scope": "mouse cortex, enhancer-AAV cell-subclass labeling toolkit",
  "claim_text": "Cell-subclass-specific enhancers identified from single-cell chromatin-accessibility data and cloned into AAVs drive transgene expression in specific mouse cortical projection-neuron subclasses, and combined with a new triple-reporter Ai213 mouse, enable simultaneous labelling of three neuronal classes/subclasses in a single transgenic animal.",
  "raw_fields": {
    "n": null,
    "doi": "10.1016/j.neuron.2021.03.011",
    "claim": "Cell-subclass-specific enhancers identified from single-cell chromatin-accessibility data and cloned into AAVs drive transgene expression in specific mouse cortical projection-neuron subclasses, and combined with a new triple-reporter Ai213 mouse, enable simultaneous labelling of three neuronal classes/subclasses in a single transgenic animal.",
    "cite_key": "Graybuck2021",
    "evidence": "Single-cell chromatin accessibility profiling, enhancer cloning into AAV, and intersectional combination with the Ai213 triple-reporter mouse.",
    "effect_size": null,
    "text_access": "abstract_only",
    "study_system": "mouse cortex, enhancer-AAV cell-subclass labeling toolkit",
    "argument_role": "supporting",
    "replication_status": "replication_unknown",
    "claim_source_sentence": "When cloned into recombinant adeno-associated viruses (AAVs) and delivered to the brain, these enhancers drive transgene expression in specific cortical cell subclasses. We extensively characterized several enhancer AAVs to show that they label different projection neuron subclasses... coupling enhancer viruses expressing recombinases to a newly generated transgenic mouse, Ai213, enables strong labeling of three different neuronal classes/subclasses in the brain of a single transgenic animal.",
    "source_provenance_status": "non_substring_match",
    "replication_evidence_dois": [],
    "effect_size_source_sentence": null
  },
  "section_id": "section_01",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_01_evidence_package.json",
  "effect_size": null,
  "review_repo": "ComputationalReviewRecurrence",
  "section_ref": "wiki_page:computationalreviewrecurrence-01-introduction",
  "source_kind": "review_finding",
  "source_path": "evidence/section_01_evidence_package.json",
  "source_refs": [
    "paper:paper-pm-33789083"
  ],
  "source_span": "When cloned into recombinant adeno-associated viruses (AAVs) and delivered to the brain, these enhancers drive transgene expression in specific cortical cell subclasses. We extensively characterized several enhancer AAVs to show that they label different projection neuron subclasses... coupling enhancer viruses expressing recombinases to a newly generated transgenic mouse, Ai213, enables strong labeling of three dif...",
  "study_system": "mouse cortex, enhancer-AAV cell-subclass labeling toolkit",
  "evidence_refs": [
    {
      "ref": "paper:paper-pm-33789083"
    }
  ],
  "section_title": "1. Introduction — what \"excitatory recurrence\" means operationally in mouse cortex, and why isolating it from inhibition and from clinical framings is a defensible analytic move for basic-research synthesis",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
  },
  "evidence_summary": "Single-cell chromatin accessibility profiling, enhancer cloning into AAV, and intersectional combination with the Ai213 triple-reporter mouse.",
  "review_bundle_ref": "analysis_bundle:ab-d9c479db9be9",
  "replication_status": "replication_unknown",
  "review_package_ref": "analysis_bundle:ab-d9c479db9be9",
  "source_artifact_ref": "wiki_page:computationalreviewrecurrence-01-introduction",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_01_evidence_package.json",
  "commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}