Version history

{
  "scope": "mouse cortex, in utero electroporation E15.5, slice patch-clamp",
  "claim_text": "Cell-autonomous reduction of activity in CGE-derived cortical interneurons by E15.5 in utero Kir2.1 electroporation produces subtype-specific developmental alterations: reelin+ and CR+ cells acquire abnormal laminar positioning and altered EPSC kinetics, while intrinsic firing matures more slowly across all CGE subtypes.",
  "raw_fields": {
    "n": 15,
    "doi": "10.3389/fncir.2012.00066",
    "claim": "Cell-autonomous reduction of activity in CGE-derived cortical interneurons by E15.5 in utero Kir2.1 electroporation produces subtype-specific developmental alterations: reelin+ and CR+ cells acquire abnormal laminar positioning and altered EPSC kinetics, while intrinsic firing matures more slowly across all CGE subtypes.",
    "title": null,
    "cite_key": "Karayannis2012",
    "evidence": "Body, Results: in utero electroporation of Kir2.1 at E15.5 to silence CGE-lineage interneurons, followed by patch-clamp characterization of intrinsic properties and sEPSC/sIPSC kinetics in reelin+, CR+, and VIP+ subgroups.",
    "effect_size": "Kir2.1 reduced AP discharge from 93±10 Hz to 44±8 Hz; widened AP half-width from 1.3±0.15 ms to 3±0.32 ms",
    "figure_data": [
      {
        "type": "electrophysiology",
        "unit": "Hz",
        "metric": "max AP discharge rate (Hz)",
        "source": "Results, in vitro patch clamp",
        "context": "CGE-derived interneurons P12–P15 cortex; Kir2.1 expression reduces excitability",
        "kir21_value": "44 ± 8",
        "control_value": "93 ± 10"
      },
      {
        "type": "electrophysiology",
        "unit": "ms",
        "metric": "AP half-width",
        "source": "Results, in vitro patch clamp",
        "context": "Same CGE cells; Kir2.1 broadens action potential",
        "kir21_value": "3.0 ± 0.32",
        "control_value": "1.3 ± 0.15"
      }
    ],
    "text_access": "fulltext",
    "study_system": "mouse cortex, in utero electroporation E15.5, slice patch-clamp",
    "_source_cluster": "cluster_02_development_lineage",
    "replication_status": "replicated",
    "_source_cluster_index": 6,
    "claim_source_sentence": "Indeed, reducing neuronal excitability within this population, by expressing the inward rectifying potassium channel Kir2.1 causes Re+ and Cr+ subtypes to acquire abnormal laminar positioning.",
    "replication_evidence_dois": [
      "10.1038/nature09865",
      "10.1523/jneurosci.1021-21.2021"
    ],
    "effect_size_source_sentence": "This difference likely underlies the reduced maximum discharge frequency of APs observed in Kir2.1-expressing interneurons compared to controls (Kir2.1: 44 ± 7.89 Hz, n = 9 vs. controls: 93 ± 10.06 Hz, n = 6; p = 0.0004) upon a 500 ms-long square current pulse."
  },
  "section_id": "section_03",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP/blob/95e761177f7d2ec565983d3307c14ec238f9677c/evidence/section_03_evidence_package.json",
  "effect_size": "Kir2.1 reduced AP discharge from 93±10 Hz to 44±8 Hz; widened AP half-width from 1.3±0.15 ms to 3±0.32 ms",
  "review_repo": "ComputationalReviewVIP",
  "section_ref": "wiki_page:computationalreviewvip-03-development",
  "source_kind": "review_finding",
  "source_path": "evidence/section_03_evidence_package.json",
  "source_refs": [
    "paper:paper-4f6575b19614"
  ],
  "source_span": "Indeed, reducing neuronal excitability within this population, by expressing the inward rectifying potassium channel Kir2.1 causes Re+ and Cr+ subtypes to acquire abnormal laminar positioning.",
  "study_system": "mouse cortex, in utero electroporation E15.5, slice patch-clamp",
  "evidence_refs": [
    {
      "ref": "paper:paper-4f6575b19614"
    }
  ],
  "section_title": "Developmental Origins and Postnatal Maturation",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "95e761177f7d2ec565983d3307c14ec238f9677c",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP"
  },
  "evidence_summary": "Body, Results: in utero electroporation of Kir2.1 at E15.5 to silence CGE-lineage interneurons, followed by patch-clamp characterization of intrinsic properties and sEPSC/sIPSC kinetics in reelin+, CR+, and VIP+ subgroups.",
  "review_bundle_ref": "analysis_bundle:ab-2ce40c33e827",
  "replication_status": "replicated",
  "review_package_ref": "analysis_bundle:ab-2ce40c33e827",
  "source_artifact_ref": "wiki_page:computationalreviewvip-03-development",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP/blob/95e761177f7d2ec565983d3307c14ec238f9677c/evidence/section_03_evidence_package.json",
  "commit_sha": "95e761177f7d2ec565983d3307c14ec238f9677c",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewVIP"
}