Cell-autonomous reduction of activity in CGE-derived cortical interneurons by E…

Cell-autonomous reduction of activity in CGE-derived cortical interneurons by E15.5 in utero Kir2.1 electroporation produces subtype-specific developmental alterations: reelin+ and CR+ cells acquire abnormal laminar positioning and altered EPSC kinetics, while intrinsic firing matures more slowly across all CGE subtypes.

{
  "n": 15,
  "doi": "10.3389/fncir.2012.00066",
  "claim": "Cell-autonomous reduction of activity in CGE-derived cortical interneurons by E15.5 in utero Kir2.1 electroporation produces subtype-specific developmental alterations: reelin+ and CR+ cells acquire abnormal laminar positioning and altered EPSC kinetics, while intrinsic firing matures more slowly across all CGE subtypes.",
  "title": null,
  "cite_key": "Karayannis2012",
  "evidence": "Body, Results: in utero electroporation of Kir2.1 at E15.5 to silence CGE-lineage interneurons, followed by patch-clamp characterization of intrinsic properties and sEPSC/sIPSC kinetics in reelin+, CR+, and VIP+ subgroups.",
  "effect_size": "Kir2.1 reduced AP discharge from 93±10 Hz to 44±8 Hz; widened AP half-width from 1.3±0.15 ms to 3±0.32 ms",
  "figure_data": [
    {
      "type": "electrophysiology",
      "unit": "Hz",
      "metric": "max AP discharge rate (Hz)",
      "source": "Results, in vitro patch clamp",
      "context": "CGE-derived interneurons P12–P15 cortex; Kir2.1 expression reduces excitability",
      "kir21_value": "44 ± 8",
      "control_value": "93 ± 10"
    },
    {
      "type": "electrophysiology",
      "unit": "ms",
      "metric": "AP half-width",
      "source": "Results, in vitro patch clamp",
      "context": "Same CGE cells; Kir2.1 broadens action potential",
      "kir21_value": "3.0 ± 0.32",
      "control_value": "1.3 ± 0.15"
    }
  ],
  "text_access": "fulltext",
  "study_system": "mouse cortex, in utero electroporation E15.5, slice patch-clamp",
  "_source_cluster": "cluster_02_development_lineage",
  "replication_status": "replicated",
  "_source_cluster_index": 6,
  "claim_source_sentence": "Indeed, reducing neuronal excitability within this population, by expressing the inward rectifying potassium channel Kir2.1 causes Re+ and Cr+ subtypes to acquire abnormal laminar positioning.",
  "replication_evidence_dois": [
    "10.1038/nature09865",
    "10.1523/jneurosci.1021-21.2021"
  ],
  "effect_size_source_sentence": "This difference likely underlies the reduced maximum discharge frequency of APs observed in Kir2.1-expressing interneurons compared to controls (Kir2.1: 44 ± 7.89 Hz, n = 9 vs. controls: 93 ± 10.06 Hz, n = 6; p = 0.0004) upon a 500 ms-long square current pulse."
}

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]

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