Version history

{
  "scope": "Cre-dependent ChR2/Arch/eNpHR3.0 transgenic mouse lines crossed with Emx1-Cre, PV-Cre, etc.",
  "claim_text": "The Ai27/Ai32/Ai35/Ai39 Cre-dependent knock-in lines provide robust expression of ChR2-tdTomato, ChR2-EYFP, eNpHR3.0 and Arch-ER2 in the adult mouse brain after Cre activation, enabling combinatorial cell-type-specific optogenetic activation or silencing in vivo.",
  "raw_fields": {
    "n": 0,
    "doi": "10.1038/nn.3078",
    "claim": "The Ai27/Ai32/Ai35/Ai39 Cre-dependent knock-in lines provide robust expression of ChR2-tdTomato, ChR2-EYFP, eNpHR3.0 and Arch-ER2 in the adult mouse brain after Cre activation, enabling combinatorial cell-type-specific optogenetic activation or silencing in vivo.",
    "cite_key": "Madisen2012",
    "evidence": "In vitro and in vivo light-evoked photocurrents and behavioral validation; PV-Cre crosses photoactivated specifically.",
    "effect_size": ".0",
    "text_access": "fulltext",
    "study_system": "Cre-dependent ChR2/Arch/eNpHR3.0 transgenic mouse lines crossed with Emx1-Cre, PV-Cre, etc.",
    "source_cluster_id": "cluster_10",
    "replication_status": "independently_replicated",
    "claim_source_sentence": "Cell-type-specific expression of optogenetic molecules allows temporally precise manipulation of targeted neuronal activity. Here we present a toolbox of 4 knock-in mouse lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2-tdTomato and ChR2-EYFP, halorhodopsin eNpHR3.0, and archaerhodopsin Arch-ER2.",
    "replication_evidence_dois": [
      "10.1016/j.cell.2018.06.035"
    ],
    "effect_size_source_sentence": "Cell-type-specific expression of optogenetic molecules allows temporally precise manipulation of targeted neuronal activity. Here we present a toolbox of 4 knock-in mouse lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2-tdTomato and ChR2-EYFP, halorhodopsin eNpHR3.0, and archaerhodopsin Arch-ER2."
  },
  "section_id": "section_11",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewLoops/blob/0632aae8abc141909207fe91f6349b9e36489c3b/evidence/section_11_evidence_package.json",
  "effect_size": ".0",
  "review_repo": "ComputationalReviewLoops",
  "section_ref": "wiki_page:computationalreviewloops-11",
  "source_kind": "review_finding",
  "source_path": "evidence/section_11_evidence_package.json",
  "source_refs": [
    "paper:fee52c07-8751-4bc4-aff6-6d5f77579b4e"
  ],
  "source_span": "Cell-type-specific expression of optogenetic molecules allows temporally precise manipulation of targeted neuronal activity. Here we present a toolbox of 4 knock-in mouse lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2-tdTomato and ChR2-EYFP, halorhodopsin eNpHR3.0, and archaerhodopsin Arch-ER2.",
  "study_system": "Cre-dependent ChR2/Arch/eNpHR3.0 transgenic mouse lines crossed with Emx1-Cre, PV-Cre, etc.",
  "evidence_refs": [
    {
      "ref": "paper:fee52c07-8751-4bc4-aff6-6d5f77579b4e"
    }
  ],
  "section_title": "Cell-Type-Specific Dissection of Loop Circuits (Opto/Chemo/Tracing)",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "0632aae8abc141909207fe91f6349b9e36489c3b",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewLoops"
  },
  "evidence_summary": "In vitro and in vivo light-evoked photocurrents and behavioral validation; PV-Cre crosses photoactivated specifically.",
  "review_bundle_ref": "analysis_bundle:ab-d49e54403ef9",
  "replication_status": "independently_replicated",
  "review_package_ref": "analysis_bundle:ab-d49e54403ef9",
  "source_artifact_ref": "wiki_page:computationalreviewloops-11",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewLoops/blob/0632aae8abc141909207fe91f6349b9e36489c3b/evidence/section_11_evidence_package.json",
  "commit_sha": "0632aae8abc141909207fe91f6349b9e36489c3b",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewLoops"
}