- claim_text
The Ai27/Ai32/Ai35/Ai39 Cre-dependent knock-in lines provide robust expression of ChR2-tdTomato, ChR2-EYFP, eNpHR3.0 and Arch-ER2 in the adult mouse brain after Cre activation, enabling combinatorial cell-type-specific optogenetic activation or silencing in vivo.
- raw_fields
{
"n": 0,
"doi": "10.1038/nn.3078",
"claim": "The Ai27/Ai32/Ai35/Ai39 Cre-dependent knock-in lines provide robust expression of ChR2-tdTomato, ChR2-EYFP, eNpHR3.0 and Arch-ER2 in the adult mouse brain after Cre activation, enabling combinatorial cell-type-specific optogenetic activation or silencing in vivo.",
"cite_key": "Madisen2012",
"evidence": "In vitro and in vivo light-evoked photocurrents and behavioral validation; PV-Cre crosses photoactivated specifically.",
"effect_size": ".0",
"text_access": "fulltext",
"study_system": "Cre-dependent ChR2/Arch/eNpHR3.0 transgenic mouse lines crossed with Emx1-Cre, PV-Cre, etc.",
"source_cluster_id": "cluster_10",
"replication_status": "independently_replicated",
"claim_source_sentence": "Cell-type-specific expression of optogenetic molecules allows temporally precise manipulation of targeted neuronal activity. Here we present a toolbox of 4 knock-in mouse lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2-tdTomato and ChR2-EYFP, halorhodopsin eNpHR3.0, and archaerhodopsin Arch-ER2.",
"replication_evidence_dois": [
"10.1016/j.cell.2018.06.035"
],
"effect_size_source_sentence": "Cell-type-specific expression of optogenetic molecules allows temporally precise manipulation of targeted neuronal activity. Here we present a toolbox of 4 knock-in mouse lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2-tdTomato and ChR2-EYFP, halorhodopsin eNpHR3.0, and archaerhodopsin Arch-ER2."
}- source_refs
[
"paper:fee52c07-8751-4bc4-aff6-6d5f77579b4e"
]
- source_span
Cell-type-specific expression of optogenetic molecules allows temporally precise manipulation of targeted neuronal activity. Here we present a toolbox of 4 knock-in mouse lines engineered for strong, Cre-dependent expression of channelrhodopsins ChR2-tdTomato and ChR2-EYFP, halorhodopsin eNpHR3.0, and archaerhodopsin Arch-ER2.
- evidence_refs
[
{
"ref": "paper:fee52c07-8751-4bc4-aff6-6d5f77579b4e"
}
]- source_policy
{
"mode": "public_source_pointer_with_short_context",
"notes": [
"Local review repositories are read-only inputs.",
"SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
],
"source_commit_sha": "0632aae8abc141909207fe91f6349b9e36489c3b",
"source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewLoops"
}