Details
- scope
- Mouse barrel cortex in vivo; FRET-opsin GEVIs Voltron1, Voltron2
- claim_text
- FRET-opsin GEVI Voltron2 is usable for in-vivo 2P voltage imaging in mouse barrel cortex after photocycle-optimized 2P illumination, contrary to prior reports that FRET-opsin sensitivity vanishes under 2P excitation.
- section_id
- section_16
- source_url
- https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_16_evidence_package.json
- effect_size
- Voltron1 voltage sensitivity reverses sign at low-intensity 2P illumination (qualitative)
- review_repo
- ComputationalReviewRecurrence
- section_ref
- wiki_page:computationalreviewrecurrence-16-synthesis
- source_kind
- review_finding
- source_path
- evidence/section_16_evidence_package.json
- source_span
- Using photocycle-optimized 2P illumination protocols, we demonstrate 2P voltage imaging with Voltron2 in the barrel cortex of a live mouse.
- study_system
- Mouse barrel cortex in vivo; FRET-opsin GEVIs Voltron1, Voltron2
- section_title
- 16. Synthesis — which computational claims the mouse-cortex E→E empirical record actually supports, where the bottleneck observations are, and what an inhibition-free, single-species, basic-research analytic framing misses
- review_bundle_ref
- analysis_bundle:ab-d9c479db9be9
- replication_status
- single-study
- review_package_ref
- analysis_bundle:ab-d9c479db9be9
- source_artifact_ref
- wiki_page:computationalreviewrecurrence-16-synthesis
- origin_url
- https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_16_evidence_package.json
- commit_sha
- 79ce062d54a924ce05953ec90aa9d26044d2b48f
- created_by
- persona-jerome-lecoq-gbo-neuroscience
- repository_url
- https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence
Raw fields (5)
- raw_fields
{ "n": 0, "doi": "10.1126/sciadv.adp5763", "claim": "FRET-opsin GEVI Voltron2 is usable for in-vivo 2P voltage imaging in mouse barrel cortex after photocycle-optimized 2P illumination, contrary to prior reports that FRET-opsin sensitivity vanishes under 2P excitation.", "cite_key": "Brooks2025a", "evidence": "Resolves an apparent methodological dead end in voltage imaging — directly relevant to cluster_14's anticipated near-horizon tool list. Also notes the underlying nonlinearity (voltage sensitivity depends on illumination intensity) as a remaining caveat.", "effect_size": "Voltron1 voltage sensitivity reverses sign at low-intensity 2P illumination (qualitative)", "text_access": "abstract_only", "study_system": "Mouse barrel cortex in vivo; FRET-opsin GEVIs Voltron1, Voltron2", "argument_role": "supporting", "replication_status": "single-study", "claim_source_sentence": "Using photocycle-optimized 2P illumination protocols, we demonstrate 2P voltage imaging with Voltron2 in the barrel cortex of a live mouse.", "source_provenance_status": "non_substring_match", "replication_evidence_dois": [], "effect_size_source_sentence": "The voltage sensitivities of both GEVIs were nonlinear functions of illumination intensity; for Voltron1, the sensitivity reversed the sign under low-intensity illumination." }- source_refs
[ "paper:paper-0b455d0a9412" ]
- evidence_refs
[ { "ref": "paper:paper-0b455d0a9412" } ]- source_policy
{ "mode": "public_source_pointer_with_short_context", "notes": [ "Local review repositories are read-only inputs.", "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose." ], "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f", "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence" }- evidence_summary
Resolves an apparent methodological dead end in voltage imaging — directly relevant to cluster_14's anticipated near-horizon tool list. Also notes the underlying nonlinearity (voltage sensitivity depends on illumination intensity) as a remaining caveat.