Version history

{
  "scope": "Mouse barrel cortex in vivo; FRET-opsin GEVIs Voltron1, Voltron2",
  "claim_text": "FRET-opsin GEVI Voltron2 is usable for in-vivo 2P voltage imaging in mouse barrel cortex after photocycle-optimized 2P illumination, contrary to prior reports that FRET-opsin sensitivity vanishes under 2P excitation.",
  "raw_fields": {
    "n": 0,
    "doi": "10.1126/sciadv.adp5763",
    "claim": "FRET-opsin GEVI Voltron2 is usable for in-vivo 2P voltage imaging in mouse barrel cortex after photocycle-optimized 2P illumination, contrary to prior reports that FRET-opsin sensitivity vanishes under 2P excitation.",
    "cite_key": "Brooks2025a",
    "evidence": "Resolves an apparent methodological dead end in voltage imaging — directly relevant to cluster_14's anticipated near-horizon tool list. Also notes the underlying nonlinearity (voltage sensitivity depends on illumination intensity) as a remaining caveat.",
    "effect_size": "Voltron1 voltage sensitivity reverses sign at low-intensity 2P illumination (qualitative)",
    "text_access": "abstract_only",
    "study_system": "Mouse barrel cortex in vivo; FRET-opsin GEVIs Voltron1, Voltron2",
    "argument_role": "supporting",
    "replication_status": "single-study",
    "claim_source_sentence": "Using photocycle-optimized 2P illumination protocols, we demonstrate 2P voltage imaging with Voltron2 in the barrel cortex of a live mouse.",
    "source_provenance_status": "non_substring_match",
    "replication_evidence_dois": [],
    "effect_size_source_sentence": "The voltage sensitivities of both GEVIs were nonlinear functions of illumination intensity; for Voltron1, the sensitivity reversed the sign under low-intensity illumination."
  },
  "section_id": "section_16",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_16_evidence_package.json",
  "effect_size": "Voltron1 voltage sensitivity reverses sign at low-intensity 2P illumination (qualitative)",
  "review_repo": "ComputationalReviewRecurrence",
  "section_ref": "wiki_page:computationalreviewrecurrence-16-synthesis",
  "source_kind": "review_finding",
  "source_path": "evidence/section_16_evidence_package.json",
  "source_refs": [
    "paper:paper-0b455d0a9412"
  ],
  "source_span": "Using photocycle-optimized 2P illumination protocols, we demonstrate 2P voltage imaging with Voltron2 in the barrel cortex of a live mouse.",
  "study_system": "Mouse barrel cortex in vivo; FRET-opsin GEVIs Voltron1, Voltron2",
  "evidence_refs": [
    {
      "ref": "paper:paper-0b455d0a9412"
    }
  ],
  "section_title": "16. Synthesis — which computational claims the mouse-cortex E→E empirical record actually supports, where the bottleneck observations are, and what an inhibition-free, single-species, basic-research analytic framing misses",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
  },
  "evidence_summary": "Resolves an apparent methodological dead end in voltage imaging — directly relevant to cluster_14's anticipated near-horizon tool list. Also notes the underlying nonlinearity (voltage sensitivity depends on illumination intensity) as a remaining caveat.",
  "review_bundle_ref": "analysis_bundle:ab-d9c479db9be9",
  "replication_status": "single-study",
  "review_package_ref": "analysis_bundle:ab-d9c479db9be9",
  "source_artifact_ref": "wiki_page:computationalreviewrecurrence-16-synthesis",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_16_evidence_package.json",
  "commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}