2P-FENDO-II is a fiber-bundle two-photon microscope that delivers concurrent tw…

2P-FENDO-II is a fiber-bundle two-photon microscope that delivers concurrent two-photon calcium imaging and holographic optogenetic photostimulation across a four-times-larger field of view than its predecessor in freely moving mice — demonstrated across somatosensory, visual, and cerebellar cortex with jGCaMP7s and ChRmine.

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  "claim": "2P-FENDO-II is a fiber-bundle two-photon microscope that delivers concurrent two-photon calcium imaging and holographic optogenetic photostimulation across a four-times-larger field of view than its predecessor in freely moving mice — demonstrated across somatosensory, visual, and cerebellar cortex with jGCaMP7s and ChRmine.",
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  "evidence": "Concurrent 2P imaging + holographic stim through fiber bundle in freely moving mice; demonstrated in three cortical regions.",
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Here, we present a significantly optimized 2P-FENDO-II system that achieves a four-times-larger field of view and a more homogeneous light distribution across the field of view, both for imaging and photostimulation, while achieving better flexibility and thus optimal adaptation to the study of freely moving mice.

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15. Methodological limits and emerging tools — what current mouse-cortex tools cannot yet measure about E→E recurrence (subthreshold network activity, fast plasticity in vivo, millimetre-scale dynamic connectomes), and what is on the near horizon

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