High-throughput transcriptomic and epigenomic profiling of >450,000 single nucl…

High-throughput transcriptomic and epigenomic profiling of >450,000 single nuclei from human, marmoset and mouse primary motor cortex demonstrates a broadly conserved cellular makeup whose similarities mirror evolutionary distance, while many cell-type-specific specializations (proportions, expression, DNA methylation, chromatin state) and few conserved marker genes are species-dependent.

{
  "n": 450000,
  "doi": "10.1038/s41586-021-03465-8",
  "claim": "High-throughput transcriptomic and epigenomic profiling of >450,000 single nuclei from human, marmoset and mouse primary motor cortex demonstrates a broadly conserved cellular makeup whose similarities mirror evolutionary distance, while many cell-type-specific specializations (proportions, expression, DNA methylation, chromatin state) and few conserved marker genes are species-dependent.",
  "cite_key": "Bakken2021",
  "evidence": "Cross-species snRNA-seq plus snmC-seq and ATAC-seq of primary motor cortex (M1) generated a consensus cell-type taxonomy revealing conservation that scales with phylogenetic distance and identifying species-specific gene-regulatory specializations.",
  "effect_size": ">450,000 single nuclei profiled across human, marmoset, mouse",
  "text_access": "fulltext",
  "study_system": "human, marmoset, mouse primary motor cortex; >450,000 single nuclei",
  "source_cluster_id": "cluster_14",
  "replication_status": "independently_replicated",
  "claim_source_sentence": "Here, using high-throughput transcriptomic and epigenomic profiling of more than 450,000 single nuclei in humans, marmoset monkeys and mice, we demonstrate a broadly conserved cellular makeup of this region, with similarities that mirror evolutionary distance and are consistent between the transcriptome and epigenome.",
  "replication_evidence_dois": [
    "10.1038/s41586-019-1506-7",
    "10.1126/science.ade9516"
  ],
  "effect_size_source_sentence": "Here, using high-throughput transcriptomic and epigenomic profiling of more than 450,000 single nuclei in humans, marmoset monkeys and mice, we demonstrate a broadly conserved cellular makeup of this region, with similarities that mirror evolutionary distance and are consistent between the transcriptome and epigenome."
}

[
  "paper:paper-pm-34616062"
]

Here, using high-throughput transcriptomic and epigenomic profiling of more than 450,000 single nuclei in humans, marmoset monkeys and mice, we demonstrate a broadly conserved cellular makeup of this region, with similarities that mirror evolutionary distance and are consistent between the transcriptome and epigenome.

[
  {
    "ref": "paper:paper-pm-34616062"
  }
]

{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "0632aae8abc141909207fe91f6349b9e36489c3b",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewLoops"
}

Cross-species snRNA-seq plus snmC-seq and ATAC-seq of primary motor cortex (M1) generated a consensus cell-type taxonomy revealing conservation that scales with phylogenetic distance and identifying species-specific gene-regulatory specializations.