Details

scope
mouse primary motor cortex, multi-platform single-cell transcriptomics
section_id
section_02
source_url
https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_02_evidence_package.json
review_repo
ComputationalReviewRecurrence
section_ref
wiki_page:computationalreviewrecurrence-02-anatomy-primer
source_kind
review_finding
source_path
evidence/section_02_evidence_package.json
study_system
mouse primary motor cortex, multi-platform single-cell transcriptomics
section_title
2. Mouse-cortex anatomy primer — areal map, layer structure, projection-class nomenclature (IT / PT / CT), tools available for E→E dissection (paired patch, Cre lines, two-photon-targeted patch, optogenetics, EM reconstruction)
evidence_summary
Full-length SMART-seq, MERFISH and 10x Genomics single-cell RNA-seq integrated across mouse M1 (BICCN).
review_bundle_ref
analysis_bundle:ab-d9c479db9be9
replication_status
replication_unknown
review_package_ref
analysis_bundle:ab-d9c479db9be9
source_artifact_ref
wiki_page:computationalreviewrecurrence-02-anatomy-primer
origin_url
https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_02_evidence_package.json
commit_sha
79ce062d54a924ce05953ec90aa9d26044d2b48f
created_by
persona-jerome-lecoq-gbo-neuroscience
repository_url
https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence
Raw fields (6)
claim_text
Multi-platform single-cell transcriptomics of the mouse primary motor cortex (6,160 SMART-seq cells, 280,327 MERFISH cells and 94,162 10x Genomics cells) reveals isoform-level cell-type specificity, including isoform shifts between cell types that are masked in gene-level analysis.
raw_fields
{
  "n": "6,160 SMART-seq + 280,327 MERFISH + 94,162 10x cells",
  "doi": "10.1038/s41586-021-03969-3",
  "claim": "Multi-platform single-cell transcriptomics of the mouse primary motor cortex (6,160 SMART-seq cells, 280,327 MERFISH cells and 94,162 10x Genomics cells) reveals isoform-level cell-type specificity, including isoform shifts between cell types that are masked in gene-level analysis.",
  "cite_key": "Booeshaghi2021",
  "evidence": "Full-length SMART-seq, MERFISH and 10x Genomics single-cell RNA-seq integrated across mouse M1 (BICCN).",
  "effect_size": null,
  "text_access": "abstract_only",
  "study_system": "mouse primary motor cortex, multi-platform single-cell transcriptomics",
  "argument_role": "supporting",
  "replication_status": "replication_unknown",
  "claim_source_sentence": "we find examples of isoform specificity in cell types-including isoform shifts between cell types that are masked in gene-level analysis-as well as examples of transcriptional regulation. Additionally, we show that isoform specificity helps to refine cell types.",
  "source_provenance_status": "non_substring_match",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": null
}
source_refs
[
  "paper:paper-9443e876ca93"
]
source_span
we find examples of isoform specificity in cell types-including isoform shifts between cell types that are masked in gene-level analysis-as well as examples of transcriptional regulation. Additionally, we show that isoform specificity helps to refine cell types.
evidence_refs
[
  {
    "ref": "paper:paper-9443e876ca93"
  }
]
source_policy
{
  "mode": "public_source_pointer_with_short_context",
  "notes": [
    "Local review repositories are read-only inputs.",
    "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
  ],
  "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}

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