Mayer et al. (2018) profiled 5,622 MGE + 7,401 CGE + 8,543 LGE single cells via…

Mayer et al. (2018) profiled 5,622 MGE + 7,401 CGE + 8,543 LGE single cells via Drop-seq with average 1,626 UMI/cell, plus 400 Dlx6a-Cre;RCE-negative GE cells via Smart-seq2 (deeper sequencing) for mitotic-progenitor enrichment, and additional 10x Genomics datasets from Lhx6-GFP-sorted MGE/CGE for cross-validation — establishing a multi-platform single-cell developmental atlas of mouse ganglionic eminences.

{
  "n": 21566,
  "doi": "10.1038/nature25999",
  "claim": "Mayer et al. (2018) profiled 5,622 MGE + 7,401 CGE + 8,543 LGE single cells via Drop-seq with average 1,626 UMI/cell, plus 400 Dlx6a-Cre;RCE-negative GE cells via Smart-seq2 (deeper sequencing) for mitotic-progenitor enrichment, and additional 10x Genomics datasets from Lhx6-GFP-sorted MGE/CGE for cross-validation — establishing a multi-platform single-cell developmental atlas of mouse ganglionic eminences.",
  "title": null,
  "cite_key": "Mayer2018",
  "evidence": "Drop-seq, Smart-seq2 (custom), and 10x Genomics scRNA-seq across mouse E13.5/E14.5 ganglionic eminences and FACS-sorted Lhx6+/Lhx6- populations.",
  "effect_size": "21,566+ Drop-seq GE cells + 400 Smart-seq2 + 10x validation",
  "figure_data": [
    {
      "type": "sample_count",
      "unit": "cells",
      "value": 5622,
      "metric": "Drop-seq cells profiled from mouse MGE",
      "source": "Mayer 2018 Drop-seq",
      "context": "E13.5"
    },
    {
      "type": "sample_count",
      "unit": "cells",
      "value": 7401,
      "metric": "Drop-seq cells profiled from mouse CGE",
      "source": "Mayer 2018 Drop-seq",
      "context": "E14.5"
    },
    {
      "type": "sample_count",
      "unit": "cells",
      "value": 8543,
      "metric": "Drop-seq cells profiled from mouse LGE",
      "source": "Mayer 2018 Drop-seq",
      "context": "E14.5"
    }
  ],
  "text_access": "fulltext",
  "study_system": "mouse, GE single-cell sequencing (Drop-seq+Smart-seq2+10x)",
  "_source_cluster": "cluster_02_development_lineage",
  "replication_status": "primary",
  "_source_cluster_index": 115,
  "claim_source_sentence": "After cell dissociation, we utilized Drop-seq 9 to sequence the transcriptomes of 5,622 single cells from the MGE, 7,401 from the CGE, and 8,543 from the LGE, from replicate experiments, observing on average 1626 UMI/cell.",
  "replication_evidence_dois": [],
  "effect_size_source_sentence": "After cell dissociation, we utilized Drop-seq 9 to sequence the transcriptomes of 5,622 single cells from the MGE, 7,401 from the CGE, and 8,543 from the LGE, from replicate experiments, observing on average 1626 UMI/cell."
}

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]

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