- claim_text
A new positive-going hybrid voltage indicator HVI+-Cy3b shows whole-cell fluorescence increases of 55.0 ± 2.1% ΔF/F0 per 100 mV with millisecond kinetics, enabling lower-background voltage imaging of action potentials and providing an alternative to negative-going voltage indicators used in mouse cortex.
- raw_fields
{
"n": 0,
"doi": "10.1126/sciadv.ads1807",
"claim": "A new positive-going hybrid voltage indicator HVI+-Cy3b shows whole-cell fluorescence increases of 55.0 ± 2.1% ΔF/F0 per 100 mV with millisecond kinetics, enabling lower-background voltage imaging of action potentials and providing an alternative to negative-going voltage indicators used in mouse cortex.",
"cite_key": "Liu2025d",
"evidence": "Quantitative voltage-indicator development relevant to voltage imaging methodology in mouse cortex.",
"effect_size": "55.0% ΔF/F0 per 100 mV; rise time ~2.24 ms; decay ~1.33 ms",
"text_access": "fulltext",
"study_system": "Cultured neurons and cardiomyocytes; voltage indicator characterization",
"argument_role": "supporting",
"replication_status": "single-study",
"claim_source_sentence": "The highest voltage sensitivity was observed in HVI + -Cy3b, with whole-cell fluorescence increasing by 55.0 ± 2.1% Δ F / F 0 per 100 mV (fast component of rise time constant: 2.24 ± 0.38 ms; fast component of decay time constant: 1.33 ± 0.18 ms), followed by HVI + -AF488 (47.6 ± 1.6%), HVI + -COT-Cy3 (40.8 ± 1.8%), and HVI + -Cy3 (31.4 ± 1.4%).",
"source_provenance_status": "ok",
"replication_evidence_dois": [],
"effect_size_source_sentence": "The highest voltage sensitivity was observed in HVI + -Cy3b, with whole-cell fluorescence increasing by 55.0 ± 2.1% Δ F / F 0 per 100 mV (fast component of rise time constant: 2.24 ± 0.38 ms; fast component of decay time constant: 1.33 ± 0.18 ms), followed by HVI + -AF488 (47.6 ± 1.6%), HVI + -COT-Cy3 (40.8 ± 1.8%), and HVI + -Cy3 (31.4 ± 1.4%)."
}- source_refs
[
"paper:paper-0ced35200161"
]
- source_span
The highest voltage sensitivity was observed in HVI + -Cy3b, with whole-cell fluorescence increasing by 55.0 ± 2.1% Δ F / F 0 per 100 mV (fast component of rise time constant: 2.24 ± 0.38 ms; fast component of decay time constant: 1.33 ± 0.18 ms), followed by HVI + -AF488 (47.6 ± 1.6%), HVI + -COT-Cy3 (40.8 ± 1.8%), and HVI + -Cy3 (31.4 ± 1.4%).
- evidence_refs
[
{
"ref": "paper:paper-0ced35200161"
}
]- source_policy
{
"mode": "public_source_pointer_with_short_context",
"notes": [
"Local review repositories are read-only inputs.",
"SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
],
"source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
"source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}