Version history

{
  "scope": "Cultured neurons and cardiomyocytes; voltage indicator characterization",
  "claim_text": "A new positive-going hybrid voltage indicator HVI+-Cy3b shows whole-cell fluorescence increases of 55.0 ± 2.1% ΔF/F0 per 100 mV with millisecond kinetics, enabling lower-background voltage imaging of action potentials and providing an alternative to negative-going voltage indicators used in mouse cortex.",
  "raw_fields": {
    "n": 0,
    "doi": "10.1126/sciadv.ads1807",
    "claim": "A new positive-going hybrid voltage indicator HVI+-Cy3b shows whole-cell fluorescence increases of 55.0 ± 2.1% ΔF/F0 per 100 mV with millisecond kinetics, enabling lower-background voltage imaging of action potentials and providing an alternative to negative-going voltage indicators used in mouse cortex.",
    "cite_key": "Liu2025d",
    "evidence": "Quantitative voltage-indicator development relevant to voltage imaging methodology in mouse cortex.",
    "effect_size": "55.0% ΔF/F0 per 100 mV; rise time ~2.24 ms; decay ~1.33 ms",
    "text_access": "fulltext",
    "study_system": "Cultured neurons and cardiomyocytes; voltage indicator characterization",
    "argument_role": "supporting",
    "replication_status": "single-study",
    "claim_source_sentence": "The highest voltage sensitivity was observed in HVI + -Cy3b, with whole-cell fluorescence increasing by 55.0 ± 2.1% Δ F / F 0 per 100 mV (fast component of rise time constant: 2.24 ± 0.38 ms; fast component of decay time constant: 1.33 ± 0.18 ms), followed by HVI + -AF488 (47.6 ± 1.6%), HVI + -COT-Cy3 (40.8 ± 1.8%), and HVI + -Cy3 (31.4 ± 1.4%).",
    "source_provenance_status": "ok",
    "replication_evidence_dois": [],
    "effect_size_source_sentence": "The highest voltage sensitivity was observed in HVI + -Cy3b, with whole-cell fluorescence increasing by 55.0 ± 2.1% Δ F / F 0 per 100 mV (fast component of rise time constant: 2.24 ± 0.38 ms; fast component of decay time constant: 1.33 ± 0.18 ms), followed by HVI + -AF488 (47.6 ± 1.6%), HVI + -COT-Cy3 (40.8 ± 1.8%), and HVI + -Cy3 (31.4 ± 1.4%)."
  },
  "section_id": "section_16",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_16_evidence_package.json",
  "effect_size": "55.0% ΔF/F0 per 100 mV; rise time ~2.24 ms; decay ~1.33 ms",
  "review_repo": "ComputationalReviewRecurrence",
  "section_ref": "wiki_page:computationalreviewrecurrence-16-synthesis",
  "source_kind": "review_finding",
  "source_path": "evidence/section_16_evidence_package.json",
  "source_refs": [
    "paper:paper-0ced35200161"
  ],
  "source_span": "The highest voltage sensitivity was observed in HVI + -Cy3b, with whole-cell fluorescence increasing by 55.0 ± 2.1% Δ F / F 0 per 100 mV (fast component of rise time constant: 2.24 ± 0.38 ms; fast component of decay time constant: 1.33 ± 0.18 ms), followed by HVI + -AF488 (47.6 ± 1.6%), HVI + -COT-Cy3 (40.8 ± 1.8%), and HVI + -Cy3 (31.4 ± 1.4%).",
  "study_system": "Cultured neurons and cardiomyocytes; voltage indicator characterization",
  "evidence_refs": [
    {
      "ref": "paper:paper-0ced35200161"
    }
  ],
  "section_title": "16. Synthesis — which computational claims the mouse-cortex E→E empirical record actually supports, where the bottleneck observations are, and what an inhibition-free, single-species, basic-research analytic framing misses",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
  },
  "evidence_summary": "Quantitative voltage-indicator development relevant to voltage imaging methodology in mouse cortex.",
  "review_bundle_ref": "analysis_bundle:ab-d9c479db9be9",
  "replication_status": "single-study",
  "review_package_ref": "analysis_bundle:ab-d9c479db9be9",
  "source_artifact_ref": "wiki_page:computationalreviewrecurrence-16-synthesis",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_16_evidence_package.json",
  "commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}