Version history

{
  "scope": "Mouse cortex/LC; barcode-sequencing single-neuron projection mapping.",
  "claim_text": "MAPseq enables high-throughput single-neuron projection mapping in the mouse brain by labelling neurons with random RNA barcodes and sequencing axon contents from dissected target regions, scaling single-cell projection analysis to thousands of neurons.",
  "raw_fields": {
    "n": null,
    "doi": "10.1016/j.neuron.2016.07.036",
    "claim": "MAPseq enables high-throughput single-neuron projection mapping in the mouse brain by labelling neurons with random RNA barcodes and sequencing axon contents from dissected target regions, scaling single-cell projection analysis to thousands of neurons.",
    "cite_key": "Kebschull2016",
    "evidence": "Sindbis virus-delivered random RNA barcodes; barcode mRNA extracted from dissected target regions and sequenced to recover single-cell projection patterns.",
    "effect_size": "Demonstrated on mouse locus coeruleus and cortical targets; recovers preferred cortical target patterns at thousands-of-neurons scale.",
    "text_access": "fulltext",
    "study_system": "Mouse cortex/LC; barcode-sequencing single-neuron projection mapping.",
    "argument_role": "supporting",
    "replication_status": "replicated",
    "claim_source_sentence": "Here we describe MAPseq (Multiplexed Analysis of Projections by Sequencing), a technique that can map the projections of thousands or even millions of single neurons by labeling large sets of neurons with random RNA sequences (\"barcodes\").",
    "source_provenance_status": "ok",
    "replication_evidence_dois": [
      "10.1016/j.cell.2019.09.023",
      "10.1038/nature26159"
    ],
    "effect_size_source_sentence": "Applying MAPseq to the locus coeruleus (LC), we find that individual LC neurons have preferred cortical targets."
  },
  "section_id": "section_08",
  "source_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_08_evidence_package.json",
  "effect_size": "Demonstrated on mouse locus coeruleus and cortical targets; recovers preferred cortical target patterns at thousands-of-neurons scale.",
  "review_repo": "ComputationalReviewRecurrence",
  "section_ref": "wiki_page:computationalreviewrecurrence-08-cross-areal",
  "source_kind": "review_finding",
  "source_path": "evidence/section_08_evidence_package.json",
  "source_refs": [
    "paper:paper-f6fa8c802d45"
  ],
  "source_span": "Here we describe MAPseq (Multiplexed Analysis of Projections by Sequencing), a technique that can map the projections of thousands or even millions of single neurons by labeling large sets of neurons with random RNA sequences (\"barcodes\").",
  "study_system": "Mouse cortex/LC; barcode-sequencing single-neuron projection mapping.",
  "evidence_refs": [
    {
      "ref": "paper:paper-f6fa8c802d45"
    }
  ],
  "section_title": "8. Cross-areal mouse cortico-cortical excitatory connectivity — hierarchical feedforward and feedback as recurrent loops at the network level; Allen Mouse Connectivity Atlas anchored views",
  "source_policy": {
    "mode": "public_source_pointer_with_short_context",
    "notes": [
      "Local review repositories are read-only inputs.",
      "SciDEX stores paper metadata, structured evidence, file pointers, and short citation contexts; it does not copy full review prose."
    ],
    "source_commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
    "source_repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
  },
  "evidence_summary": "Sindbis virus-delivered random RNA barcodes; barcode mRNA extracted from dissected target regions and sequenced to recover single-cell projection patterns.",
  "review_bundle_ref": "analysis_bundle:ab-d9c479db9be9",
  "replication_status": "replicated",
  "review_package_ref": "analysis_bundle:ab-d9c479db9be9",
  "source_artifact_ref": "wiki_page:computationalreviewrecurrence-08-cross-areal",
  "origin_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence/blob/79ce062d54a924ce05953ec90aa9d26044d2b48f/evidence/section_08_evidence_package.json",
  "commit_sha": "79ce062d54a924ce05953ec90aa9d26044d2b48f",
  "created_by": "persona-jerome-lecoq-gbo-neuroscience",
  "repository_url": "https://github.com/AllenNeuralDynamics/ComputationalReviewRecurrence"
}