MAPseq enables high-throughput single-neuron projection mapping in the mouse br…

MAPseq enables high-throughput single-neuron projection mapping in the mouse brain by labelling neurons with random RNA barcodes and sequencing axon contents from dissected target regions, scaling single-cell projection analysis to thousands of neurons.

{
  "n": null,
  "doi": "10.1016/j.neuron.2016.07.036",
  "claim": "MAPseq enables high-throughput single-neuron projection mapping in the mouse brain by labelling neurons with random RNA barcodes and sequencing axon contents from dissected target regions, scaling single-cell projection analysis to thousands of neurons.",
  "cite_key": "Kebschull2016",
  "evidence": "Sindbis virus-delivered random RNA barcodes; barcode mRNA extracted from dissected target regions and sequenced to recover single-cell projection patterns.",
  "effect_size": "Demonstrated on mouse locus coeruleus and cortical targets; recovers preferred cortical target patterns at thousands-of-neurons scale.",
  "text_access": "fulltext",
  "study_system": "Mouse cortex/LC; barcode-sequencing single-neuron projection mapping.",
  "argument_role": "supporting",
  "replication_status": "replicated",
  "claim_source_sentence": "Here we describe MAPseq (Multiplexed Analysis of Projections by Sequencing), a technique that can map the projections of thousands or even millions of single neurons by labeling large sets of neurons with random RNA sequences (\"barcodes\").",
  "source_provenance_status": "ok",
  "replication_evidence_dois": [
    "10.1016/j.cell.2019.09.023",
    "10.1038/nature26159"
  ],
  "effect_size_source_sentence": "Applying MAPseq to the locus coeruleus (LC), we find that individual LC neurons have preferred cortical targets."
}

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