Single-cell transcriptomics, fate mapping and patch-seq reveal that cortical ne…

Single-cell transcriptomics, fate mapping and patch-seq reveal that cortical neurogliaform cells (NGCs) emerge from multiple ganglionic-eminence origins (MGE, CGE, preoptic) with distinct developmental trajectories—NGC diversity is established embryonically by lineage of origin rather than purely postnatal local cues.

{
  "n": null,
  "doi": "10.1242/dev.201830",
  "year": "2023",
  "claim": "Single-cell transcriptomics, fate mapping and patch-seq reveal that cortical neurogliaform cells (NGCs) emerge from multiple ganglionic-eminence origins (MGE, CGE, preoptic) with distinct developmental trajectories—NGC diversity is established embryonically by lineage of origin rather than purely postnatal local cues.",
  "title": "Developmental emergence of cortical neurogliaform cell diversity.",
  "authors": "Gomez L, et al.",
  "journal": "Development (Cambridge, England)",
  "cite_key": "Gomez2023",
  "effect_size": null,
  "text_access": "fulltext",
  "_source_cluster": "cluster_14_cge_lineage_context",
  "evidence_source": "fulltext",
  "cluster_relevance": "cge_lineage_context",
  "fulltext_available": true,
  "replication_status": null,
  "_source_cluster_index": 16,
  "claim_source_sentence": "GABAergic interneurons are key regulators of cortical circuit function. Among the dozens of reported transcriptionally distinct subtypes of cortical interneurons, neurogliaform cells (NGCs) are unique: they are recruited by long-range excitatory inputs, are a source of slow cortical inhibition and are able to modulate the activity of large neuronal populations. Despite their functional relevance, the developmental emergence and diversity of NGCs remains unclear. Here, by combining single-cell transcriptomics, genetic fate mapping, and electrophysiological and morphological characterization, we reveal that discrete molecular subtypes of NGCs, with distinctive anatomical and molecular profiles, populate the mouse neocortex. Furthermore, we show that NGC subtypes emerge gradually through development, as incipient discriminant molecular signatures are apparent in preoptic area (POA)-born NGC precursors. By identifying NGC developmentally conserved transcriptional programs, we report that the transcription factor Tox2 constitutes an identity hallmark across NGC subtypes. Using CRISPR-Cas9-mediated genetic loss of function, we show that Tox2 is essential for NGC development: POA-born cel"
}

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GABAergic interneurons are key regulators of cortical circuit function. Among the dozens of reported transcriptionally distinct subtypes of cortical interneurons, neurogliaform cells (NGCs) are unique: they are recruited by long-range excitatory inputs, are a source of slow cortical inhibition and are able to modulate the activity of large neuronal populations. Despite their functional relevance, the developmental e...

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